Anti-Symmetric Dimethyl-Histone H4 (Arg3) Rabbit Monoclonal Antibody [Clone RM459], 31-1351-00

Key features and details

  • Uniprot: P62805
  • Gene ID: 121504
  • Host Species: Rabbit
  • Species Reactivity: Human
  • Concentration: 1 mg/mL
  • Brand:
CAT.NO. : 31-1351-00
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Product Details
Product Details
Product NameAnti-Symmetric Dimethyl-Histone H4 (Arg3) Rabbit Monoclonal Antibody, Clone RM459
Product DescriptionRabbit monoclonal to Symmetric Dimethyl-Histone H4 (Arg3)
Catalog No.31-1351-00
Clone NameRM459
Product Data SheetAnti-Symmetric Dimethyl-Histone H4 (Arg3) Rabbit Monoclonal Antibody Data Sheet RM459
SpecificityRM459 reacts to Histone H4 symmetric dimethylated at Arginine 3 (R3me2s). No cross reactivity with unmodified Arginine 3, monomethylated or asymmetric dimethylated Arginine 3, or other methylated Arginine in Histone H4.
ImmunogenA symmetric dimethyl-peptide corresponding to the symmetric demethylated Histone H4 (Arg3).
ApplicationWestern Blot
Luminex
Species ReactivityHuman
Vertebrates
ConjugateNone
Intended UseResearch Use Only
.
Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size100 µg
Concentration 1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageWB: 0.1 µg/mL – 2 µg/mL;
Luminex: 0.001 µg/mL – 0.1 µg/mL.

Keywords
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Product Images

Luminex analysis of Designer Recombinant Nucleosomes (dNucs)
with RM459. Biotinylated dNucs with unmodified or different modified
arginine pre-bind to Luminex beads coated with streptavidin.The
beads were then incubated with 1.6 ng/mL of RM459, follow by
PE-conjugated goat anti-rabbit IgG secondary antibody.





Western Blot of acid extracts of K562 cells (1) and recombinant
histone H4 (2), using Anti-Symmetric Dimethyl-Histone H4 (Arg3)
Rabbit Monoclonal Antibody, Clone RM459, at 0.1 µg/mL.

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