anti-TXNDC5 / EndoPDI antibody

Key features and details

  • 产品描述: Goat Polyclonal antibody recognizes TXNDC5 / EndoPDI
  • 反应物种: Hu
  • 应用: FACS, ICC/IF, IHC-P, WB
  • 特异性: This antibody is expected to be able to recognise both reported human isoforms, as represented by NP_110437.2; NP_001139021.1.
  • 宿主: Goat
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: TXNDC5 / EndoPDI
  • 抗原物种: Human
  • Brand:
CAT.NO. : ARG63498
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Product Details
概述
产品描述Goat Polyclonal antibody recognizes TXNDC5 / EndoPDI
反应物种Hu
应用FACS, ICC/IF, IHC-P, WB
特异性This antibody is expected to be able to recognise both reported human isoforms, as represented by NP_110437.2; NP_001139021.1.
宿主Goat
克隆Polyclonal
同位型IgG
靶点名称TXNDC5 / EndoPDI
抗原物种Human
抗原C-SLHRFVLSQAKDEL
偶联标记Un-conjugated
別名HCC2; ERp46; Thioredoxin domain-containing protein 5; HCC-2; Thioredoxin-like protein p46; UNQ364; ER protein 46; ERP46; PDIA15; ENDOPDI; STRF8; Endoplasmic reticulum resident protein 46
应用说明
应用建议
应用推荐稀释比
FACS10 µg/ml
ICC/IF10 µg/ml
IHC-P3 - 5 µg/ml
WB0.01 - 0.1 µg/ml
应用说明WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
缓冲液Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
抗菌剂0.02% Sodium azide
稳定剂0.5% BSA
浓度0.5 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 81567 Human TXNDC5

Swiss-port # Q8NBS9 Human Thioredoxin domain-containing protein 5

背景介绍This gene encodes a protein-disulfide isomerase. Its expression is induced by hypoxia and its role may be to protect hypoxic cells from apoptosis. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring upstream MUTED (muted homolog) gene. [provided by RefSeq, Dec 2010]
研究领域Cell Biology and Cellular Response antibody; Cell Death antibody; Signaling Transduction antibody
预测分子量48 kDa
检测图片 (6)
  • ARG63498 anti-TXNDC5 / EndoPDI antibody WB image

    Western blot: Human placenta lysate (RIPA buffer, 35 µg total protein per lane) stained with ARG63498 anti-TXNDC5 / EndoPDI antibody at 0.1 µg/ml dilution.

  • ARG63498 anti-TXNDC5 / EndoPDI antibody ICC/IF image

    Immunofluorescence: Paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton. Cells were stained with ARG63498 anti-TXNDC5 / EndoPDI antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.

  • ARG63498 anti-TXNDC5 / EndoPDI antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human small intestine tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63498 anti-TXNDC5 / EndoPDI antibody at 3.75 µg/ml dilution followed by AP-staining.

  • ARG63498 anti-TXNDC5 / EndoPDI antibody WB image

    Western blot: 35 µg of HEK293 (A), A549 (B), HeLa (C) and HepG2 (D) cell lysates (in RIPA buffer) stained with ARG63498 anti-TXNDC5 / EndoPDI antibody at 0.1 µg/ml (A-C) and 0.01 µg/ml (D) dilutions and incubated at RT for 1 hour.

  • ARG63498 anti-TXNDC5 / EndoPDI antibody FACS image

    Flow Cytometry: Paraformaldehyde-fixed HeLa cells permeabilized with 0.5% Triton. Cells were stained with ARG63498 anti-TXNDC5 / EndoPDI antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).

  • ARG63498 anti-TXNDC5 / EndoPDI antibody ICC/IF image

    Immunofluorescence: Paraformaldehyde fixed U2OS cells permeabilized with 0.15% Triton. Cells were stained with ARG63498 anti-TXNDC5 / EndoPDI antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.

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