HRP-conjugated Goat IgG(H+L) Rabbit Polyclonal antibody
Key features and details
- Reactivity:Goat
- Application:WB, ELISA
- Host:Rabbit
- Clonality:Polyclonal
- Conjugate: HRP
- Target:Goat IgG(H+L)
-
Brand:
CAT.NO. : ARA6466
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Product Details
Background
Anti-Goat secondary antibodies are affinity-purified antibodies with well-characterized specificity for goat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
Application
|
Application |
Dilution Ratio |
|
WB |
1:2000 - 1:20,000 |
|
ELISA |
1:1000 - 1:20,000 |
Overview
|
Concentration |
0.2 mg/mL |
|
Host Species |
Rabbit |
|
Species Reactivity |
Goat |
|
Physical State |
Liquid |
|
Purity |
The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. |
|
Storage Buffer |
0.01 M Sodium phosphate, 0.25 M NaCl, 50% glycerol, 3 mg/ml BSA, pH 7.6. |
|
Stabilizer |
3 mg/ml BSA |
|
Clonality |
Polyclonal |
|
RRID |
AB_2864335 |
Data
IgG proteins of various species were subjected to SDS PAGE followed by western blot with HRP-conjugated Goat IgG(H+L) Rabbit antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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