Human CD163 ELISA Kit
Key features and details
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- 反应物种:
- 应用:
- 特异性:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
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Brand:
CAT.NO. : ARG81441
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81441-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81441-002 | Standard | 2 X 100 ng/vial | 4°C |
| ARG81441-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81441-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81441-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81441-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81441-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81441-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81441-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81441-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81441-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81441 Human CD163 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD163 in serum, plasma (heparin) and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | CD163 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 0.78 ng/ml |
| 样品类型 | Serum, plasma (heparin) and cell culture supernatants. |
| 标准范围 | 1.56 - 100 ng/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.2% Inter-Assay CV: 6.1% |
| 別名 | sCD163; M130; Scavenger receptor cysteine-rich type 1 protein M130; MM130; CD antigen CD163; Hemoglobin scavenger receptor |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # Q86VB7 Human Scavenger receptor cysteine-rich type 1 protein M130 |
|---|---|
| 基因名称 | CD163 |
| 全名 | CD163 molecule |
| 背景介绍 | CD163 protein is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011] |
| 生物功能 | CD163: Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells. After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions. [UniProt] |
| 产品亮点 | Related products: CD163 antibodies; CD163 ELISA Kits; CD163 Duos / Panels; Related news: New antibody panels and duos for Tumor immune microenvironment Anti-SerpinB9 therapy, a new strategy for cancer therapy RIP1 activation and pathogenesis of NASH New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | A soluble form (sCD163) is produced by proteolytic shedding which can be induced by lipopolysaccharide, phorbol ester and Fc region of immunoglobulin gamma. This cleavage is dependent on protein kinase C and tyrosine kinases and can be blocked by protease inhibitors. The shedding is inhibited by the tissue inhibitor of metalloproteinase TIMP3, and thus probably induced by membrane-bound metalloproteinases ADAMs. Phosphorylated. [UniProt] |
检测图片 (1)
ARG81441 Human CD163 ELISA Kit standard curve image
ARG81441 Human CD163 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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