Human IL18 binding protein ELISA Kit
Key features and details
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- 反应物种:
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- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
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Brand:
CAT.NO. : ARG81544
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81544-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81544-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81544-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81544-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81544-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81544-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81544-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81544-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81544-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81544-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81544-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81544 Human IL18 binding protein ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human IL18 binding protein in serum, plasma (heparin, EDTA), urine and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | IL18 binding protein |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 46.85 pg/ml |
| 样品类型 | Serum, plasma (heparin, EDTA), urine and cell culture supernatants. |
| 标准范围 | 93.7 - 6000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.4% Inter-Assay CV: 6.7% |
| 別名 | IL18BPa; Tadekinig-alfa; IL-18BP; Interleukin-18-binding protein |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | IL18BP |
| 全名 | interleukin 18 binding protein |
| 背景介绍 | The protein encoded by this gene functions as an inhibitor of the proinflammatory cytokine, IL18. It binds IL18, prevents the binding of IL18 to its receptor, and thus inhibits IL18-induced IFN-gamma production, resulting in reduced T-helper type 1 immune responses. This protein is constitutively expressed and secreted in mononuclear cells. Elevated level of this protein is detected in the intestinal tissues of patients with Crohn's disease. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Feb 2011] |
| 生物功能 | Isoform A binds to IL-18 and inhibits its activity. Functions as an inhibitor of the early TH1 cytokine response. [UniProt] |
| 产品亮点 | Related products: IL18 binding protein antibodies; IL18 binding protein ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | N- and O-glycosylated. O-glycosylated with core 1-like and core 2-like glycans. O-glycan heterogeneity at Ser-53: HexHexNAc (major) and Hex2HexNAc2 (minor). N-glycan heterogeneity at Asn-103: Hex5HexNAc4 (minor), dHex1Hex5HexNAc4 (major) and Hex6HexNAc5 (minor); N-glycan at Asn-147: dHex1Hex5HexNAc4. [UniProt] |
检测图片 (1)
ARG81544 Human IL18 binding protein ELISA Kit standard curve image
ARG81544 Human IL18 binding protein ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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