Human von Willebrand Factor ELISA Kit
Protocol Booklet
Key features and details
- Specification:
- Sensitivity:
- Standard Curve Range:
- Standard Curve Gradient:
- Number of Incubations:
- Sample Volume:
- Assay type:
- Operation Duration:
-
Brand:
Product Details
|
ng/ml |
O.D. |
Average |
Corrected |
|
|
0.00 |
0.0064 |
0.0058 |
0.0061 |
|
|
4.69 |
0.0354 |
0.0345 |
0.0350 |
0.0293 |
|
9.38 |
0.0859 |
0.0789 |
0.0824 |
0.0798 |
|
18.75 |
0.2116 |
0.1990 |
0.2053 |
0.2055 |
|
37.50 |
0.5367 |
0.5317 |
0.5342 |
0.5306 |
|
75.00 |
1.3890 |
1.3180 |
1.3535 |
1.3829 |
|
150.00 |
2.5750 |
2.7090 |
2.6420 |
2.5689 |
|
300.00 |
3.6260 |
3.6480 |
3.6370 |
3.6199 |
|
Intra-assay Precision |
|
Inter-assay Precision |
|||||
|
Sample Number |
S1 |
S2 |
S3 |
S1 |
S2 |
S3 |
|
|
22 |
22 |
22 |
6 |
6 |
6 |
||
|
Average（ng/ml） |
5.7 |
32.8 |
151.8 |
5.3 |
31.3 |
113.6 |
|
|
Standard deviation |
0.2 |
1.7 |
10.4 |
0.1 |
2.0 |
7.7 |
|
|
Coefficient of variation（%） |
2.7 |
5.2 |
6.8 |
2.6 |
6.4 |
6.7 |
|
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
The recovery ranged from 94% to 126% with an overall mean recovery of 109%.
|
Sample Matrix |
Sample Evaluated |
Range (ng/ml) |
Detectable % |
Mean of Detectable (ng/ml) |
|
Serum |
30 |
10.53-1936.87 |
100 |
1056.08 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of von Willebrand Factor in this assay. No medical histories were available for the donors.
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