Lambda Light Chains Rabbit Monoclonal Antibody(ARB981)

Key features and details

Target: Lambda Light Chains
Clone ID: ARB981
Host: Rabbit
Molecular Weight: 23 kDa
Purity: ProA affinity purified IgG
Species Cross-reactivity: Human
Applications: IHC-P
Swissprot ID: P0CG04

Brand:

CAT.NO. : ARB6773

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Size:

1mL

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Product Details

Background

Lambda light chains are polypeptide chains located in the cell membrane and cytoplasmic regions of normal B cells and plasma cells. The combination of lambda light chains and heavy chains forms immunoglobulin molecules. There are two classes of light chains found in immunoglobulins, kappa light chains and lambda light chains. Light chain production by lymphoid cells is genetically restricted such that the immunoglobulin molecules produced by an individual cell will only contain a single light chain class. The normal human kappa/lambda ratio is approximately 2:1. The presence of clear cut light chain restriction with a kappa/lambda ratio more than 10:1 is consistent with a malignant proliferation.

This antibody recognizes lambda light chains of human immunoglobulins, which may be useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin’s lymphomas. The most common feature of these malignancies is the restricted expression of a single light chain class.

Application

Application	Dilution Ratio
IHC	1:100 - 1:200

Overview

Predicted Molecular Wt	23kDa
Species Cross-reactivity	Human
Applications	IHC-P
Purity	ProA affinity purified IgG
Form	Liquid
Swissprot ID	P0CG04
Subcellular location	Cytoplasm
Recommended method	Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT (18℃-25℃) for 30 minutes
Immunogen	Synthetic peptide corresponding to residues within N-terminus of immunoglobulin lambda constant 1 was used as an immunogen
Storage Buffer	PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%