Mouse IFN alpha-1 ELISA Kit
Key features and details
- Specification:
- Sensitivity:
- Standard Curve Range:
- Standard Curve Gradient:
- Number of Incubations:
- Sample Volume:
- Assay type:
- Operation Duration:
-
Brand:
CAT.NO. : AEM0049
RMB Please choose
RMB Please choose
Size:
Trail, Bulk size or Custom requests Please contact us
Product Details
Background
Interferon-alpha (IFN-alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. IFN-alpha has both anti-viral and immunomodulatory activities on target cells.
Typical data
|
pg/ml |
O.D. |
Average |
Corrected |
|
|
0.00 |
0.0255 |
0.0288 |
0.0272 |
|
|
78.13 |
0.1023 |
0.1111 |
0.1067 |
0.0796 |
|
156.25 |
0.1886 |
0.1889 |
0.1888 |
0.1616 |
|
312.50 |
0.3705 |
0.3604 |
0.3655 |
0.3383 |
|
625.00 |
0.7418 |
0.7227 |
0.7323 |
0.7051 |
|
1250.00 |
1.4051 |
1.4527 |
1.4289 |
1.4018 |
|
2500.00 |
2.7303 |
2.5847 |
2.6575 |
2.6304 |
|
5000.00 |
4.0742 |
4.1814 |
4.1278 |
4.1007 |
Precision
|
Intra-assay Precision |
Inter-assay Precision |
|||||
|
Sample Number |
S1 |
S2 |
S3 |
S1 |
S2 |
S3 |
|
22 |
22 |
22 |
6 |
6 |
6 |
|
|
Average(pg/ml) |
87.84 |
491.57 |
1544.40 |
88.73 |
480.46 |
1517.12 |
|
Standard Deviation |
4.99 |
18.68 |
66.80 |
2.53 |
15.37 |
57.77 |
|
Coefficient of Variation(%) |
5.7 |
3.8 |
4.3 |
2.8 |
3.2 |
3.8 |
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse IFN alpha-1 into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 93% to 98% with an overall mean recovery of 96%.
The recovery ranged from 93% to 98% with an overall mean recovery of 96%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-26.30 | 66.7 | 13.29 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of IFN alpha-1 in this assay. No medical histories were available for the donors.
New Products
