Mouse MPL / TPOR ELISA Kit
Key features and details
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- 偶联标记:
- 偶联标记说明:
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CAT.NO. : ARG82349
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82349-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82349-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82349-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82349-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82349-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82349-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82349-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82349-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82349-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82349-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82349-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG82349 Mouse MPL / TPOR ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse MPL / TPOR in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| 反应物种 | Ms |
| 应用 | ELISA |
| 靶点名称 | MPL / TPOR |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 78 pg/ml |
| 样品类型 | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| 标准范围 | 156 - 10000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.7% Inter-Assay CV: 6.5% |
| 別名 | Proto-oncogene c-Mpl; MPLV; CD110; Thrombopoietin receptor; C-MPL; TPO-R; Myeloproliferative leukemia protein; TPOR; THCYT2; CD antigen CD110 |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | MPL |
| 全名 | MPL proto-oncogene, thrombopoietin receptor |
| 背景介绍 | In 1990 an oncogene, v-mpl, was identified from the murine myeloproliferative leukemia virus that was capable of immortalizing bone marrow hematopoietic cells from different lineages. In 1992 the human homologue, named, c-mpl, was cloned. Sequence data revealed that c-mpl encoded a protein that was homologous with members of the hematopoietic receptor superfamily. Presence of anti-sense oligodeoxynucleotides of c-mpl inhibited megakaryocyte colony formation. The ligand for c-mpl, thrombopoietin, was cloned in 1994. Thrombopoietin was shown to be the major regulator of megakaryocytopoiesis and platelet formation. The protein encoded by the c-mpl gene, CD110, is a 635 amino acid transmembrane domain, with two extracellular cytokine receptor domains and two intracellular cytokine receptor box motifs . TPO-R deficient mice were severely thrombocytopenic, emphasizing the important role of CD110 and thrombopoietin in megakaryocyte and platelet formation. Upon binding of thrombopoietin CD110 is dimerized and the JAK family of non-receptor tyrosine kinases, as well as the STAT family, the MAPK family, the adaptor protein Shc and the receptors themselves become tyrosine phosphorylated. [provided by RefSeq, Jul 2008] |
| 生物功能 | Receptor for thrombopoietin. May represent a regulatory molecule specific for TPO-R-dependent immune responses. [UniProt] |
| 细胞定位 | Cell membrane; Single-pass type I membrane protein. Golgi apparatus. Cell surface. [UniProt] |
| 产品亮点 | Related products: New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | Ubiquitination at Lys-553 and Lys-573 targets MPL for degradation by both the lysosomal and proteasomal pathways. The E3 ubiquitin-protein ligase CBL significantly contributes to this ubiquitination. [UniProt] |
检测图片 (1)
ARG82349 Mouse MPL / TPOR ELISA Kit standard curve image
ARG82349 Mouse MPL / TPOR ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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