PAI-2 Mouse Monoclonal Antibody (E-1)
Datasheet
Key features and details
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Brand:
CAT.NO. : ARA0201
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Product Details
BACKGROUND
PAI-1 and PAI-2 (for plasminogen activator inhibitor-1 and -2) are members of the Serpin serine proteinase inhibitor family. PAI-1 and PAI-2 have been shown to regulate uPA (urokinase - type plasminogen activator) and tPA (tissue plasminogen activator), resulting in the inhibition of proteolytic activity. Members of the Serpin family generally complex with their target proteinases, then disassociate slowly into cleaved species that fold into stable inactive forms. PAI-1 can fold into the inactive state without cleavage, resulting in the latent form of PAI-1. Activity can be restored to the latent form of PAI-1 through denaturation and renaturation. PAI-2 occurs in secreted and cytosolic forms through facultative polypeptide translocation. uPa is a serine proteinase that is a member of the Trypsin family. It is responsible for the cleavage of plasminogen at the Arg - Val bond to produce plasmin. uPA consists of two chains, designated A and B. The A chain can be cleaved, resulting in low and high molecular mass forms of uPA.
Application
PAI-2 (E-1) is recommended for detection of PAI-2 of mouse, rat and human origin by Western Blotting (starting dilution 1:100, dilution range 1:100 - 1:1000), immunoprecipitation [1 - 2 µg per 100 - 500 µg of total protein (1 ml of cell lysate)], immunofluorescence (starting dilution 1:50, dilution range 1:50 - 1:500) and solid phase ELISA (starting dilution 1:30, dilution range 1:30 - 1:3000).
Data
PAI-2 (E-1). Western blot analysis of PAI-2 expression in K-562 (A), WI-38 (B), C2C12 (C), WEHI-231 (D) and A-10 (E) whole cell lysates.
PAI-2 (E-1). Western blot analysis of PAI-2 expression in K-562 (A), JAR (B) and A-431 (C) whole cell lysates.
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