Rat IL-4 ELISA Kit

Key features and details

Specification: 96 Test
Sensitivity: 17.29 pg/ml (50 μl)
Standard Curve Range: 2.74~2000 pg/ml
Standard Curve Gradient: 7 Points/3 Folds
Number of Incubations: 2
Sample Volume: 50 μl
Assay type: Sandwich Elisa
Operation Duration: 120min

Brand:

CAT.NO. : AER0003

RMB Please choose

Size:

96T

Trail, Bulk size or Custom requests Please contact us

*产品价格可能会有所调整，请以品牌方官网实时更新的价格为准，以确保准确性。

Product Details

Background

Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13 kDa ‑ 18 kDa Th2 cytokine that shows pleiotropic effects during immune responses. It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure. Human IL-4 is synthesized with a 24 aa signal sequence. Alternate splicing generates an isoform with a 16 aa internal deletion. Mature human IL-4 shares 55%, 39% and 43% aa sequence identity with bovine, mouse, and rat IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities. - IL-4 exerts its effects through two receptor complexes. The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and ‑21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils. It promotes cell proliferation, survival, and immunoglobulin class switch to IgG4 and IgE in human B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells. IL-4 plays a dominant role in the development of allergic inflammation and asthma.

Typical data

pg/ml	O.D.		Average	Corrected
0.00	0.1050	0.1040	0.1045
2.74	0.1086	0.1036	0.1061	0.0016
8.23	0.1100	0.1080	0.1090	0.0045
24.69	0.1382	0.1433	0.1408	0.0767
74.07	0.2812	0.2953	0.2883	0.2242
222.22	0.6422	0.6543	0.6483	0.5842
666.67	1.5432	1.5153	1.5293	1.4652
2000.00	3.0881	3.1932	3.1407	3.0766

Precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average(pg/ml)	33.4	172.8	557.4	32.8	175.6	561.2
Standard Deviation	1.8	8.1	26.8	2.3	12.1	34.8
Coefficient of Variation(%)	5.4	4.7	4.8	7.0	6.9	6.2

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

Spike Recovery

The spike recovery was evaluated by spiking 3 levels of rat IL-4 into health rat serum sample. The un-spiked serum was used as blank in these experiments.

The recovery ranged from 93% to 115% with an overall mean recovery of 105%.

Sample Values

Sample Matrix	Sample Evaluated	Range (pg/ml)	Detectable (%)	Mean of Detectable (pg/ml)
Serum	30	n.d.	0	0

Serum/Plasma – Thirty samples from apparently healthy rats were evaluated for the presence of IL-6 in this assay. No medical histories were available for the donors.

n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.

New Products

Human Antithrombin III ELISA Kit

S100A7 Rabbit Polyclonal Antibody

SOX17 Mouse Monoclonal Antibody(ARA897)

SS18-SSX Rabbit Monoclonal Antibody(ARM647)

SOX9 Rabbit Monoclonal Antibody(ARA829)

Spectrin Rabbit Monoclonal Antibody(ARA894)