Rat Semaphorin 3F ELISA Kit
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
- 标准范围:
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Brand:
CAT.NO. : ARG82382
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82382-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82382-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82382-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82382-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82382-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82382-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82382-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82382-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82382-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82382-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82382-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG82382 Rat Semaphorin 3F ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat Semaphorin 3F in serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
|---|---|
| 反应物种 | Rat |
| 应用 | ELISA |
| 靶点名称 | Semaphorin 3F |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 15.6 pg/ml |
| 样品类型 | Serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
| 标准范围 | 31.2 - 2000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.1% Inter-Assay CV: 6.2% |
| 別名 | Sema III/F; SEMA-IV; Semaphorin IV; SEMA4; SEMAK; Sema IV; Semaphorin-3F |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | SEMA3F |
|---|---|
| 全名 | sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F |
| 背景介绍 | The semaphorins are a family of proteins that are involved in signaling. All the family members have a secretion signal, a 500-amino acid sema domain, and 16 conserved cysteine residues (Kolodkin et al., 1993 [PubMed 8269517]). Sequence comparisons have grouped the secreted semaphorins into 3 general classes, all of which also have an immunoglobulin domain. The semaphorin III family, consisting of human semaphorin III (SEMA3A; MIM 603961), chicken collapsin, and mouse semaphorins A, D, and E, all have a basic domain at the C terminus. Chicken collapsin contributes to path finding by axons during development by inhibiting extension of growth cones (Luo et al., 1993 [PubMed 8402908]) through an interaction with a collapsin response mediator protein of relative molecular mass 62K (CRMP62) (Goshima et al., 1995 [PubMed 7637782]), a putative homolog of an axonal guidance associated UNC33 gene product (MIM 601168). SEMA3F is a secreted member of the semaphorin III family.[supplied by OMIM, Mar 2008] |
| 生物功能 | May play a role in cell motility and cell adhesion. [UniProt] |
| 细胞定位 | Secreted. [UniProt] |
| 产品亮点 | Related products: Semaphorin antibodies; Semaphorin ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
检测图片 (1)
ARG82382 Rat Semaphorin 3F ELISA Kit standard curve image
ARG82382 Rat Semaphorin 3F ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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