25-OH Vitamin D3 Matched Antibody Pair [RMH04/RM428], 31-1315-MK

Key features and details

  • Uniprot: N/A
  • Gene ID: N/A
  • Host Species: Rabbit
  • Species Reactivity: N/A
  • Concentration: 1) Capture Antibody: Clone RMH04, 100 ug at 1.0 mg/mL in 50% Glycerol/PBS and 0.09% sodium azide; 2) Detection Antibody: Biotin Clone RM428, 50 μg at 1.0 mg/mL in 50% Glycerol/PBS and 0.09% sodium azide
  • Brand:
CAT.NO. : 31-1315-MK
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Product Details
Product Details
Product Name25-OH Vitamin D3 Matched Antibody Pair [RMH04/RM428]
Product DescriptionThe 25-OH Vitamin D3 Matched Antibody Pair is specific to 25-OH Vitamin D3 and has been demonstrated through ELISA. It does not cross-react with Vitamin D3, Vitamin D2, and 25-OH Vitamin D2. This product includes capture and detection antibodies.
Catalog No.31-1315-MK
Clone NameRMH04 Anti-25-OH Vitamin D3 Rabbit/Human Chimeric Monoclonal Antibody

RM428 Biotinylated Anti-25-OH Vitamin D3/ RMH04 Complexes Rabbit Monoclonal Antibody
Product Data Sheet25-OH Vitamin D3 Matched Antibody Pair
Protocol25-OH Vitamin D3 Sanwich ELISA Protocol
SpecificityThis antibody pair detects only 25-OH Vitamin D3. It does not cross-react with Vitamin D3, Vitamin D2, and 25-OH Vitamin D2.
ApplicationSandwich ELISA, or other Sandwich Assays
Species ReactivityN/A
ConjugateRM428: Biotinylated
Intended UseResearch Use Only
Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityComponents (1) (2) Stable for 1 Year at -20.0°C from date of receipt.
Components1)Capture Antibody: Clone RMH04, 100 µg at 1.0 mg/mL in 50% Glycerol/PBS and 0.09% sodium azide;

2)Detection Antibody: Biotin Clone RM428, 50 μg at 1.0 mg/mL in 50% Glycerol/PBS and 0.09% sodium azide
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRMH04: Rabbit/Human Chimeric
RM428: Rabbit IgG
Rabbit Fv/ Human IgG CH and Cκ
Keywords
UsageELISA: Capture Antibody 50 ng/well – 200 ng/well;
Detection Antibody 0.5 µg/mL – 2 µg/mL;
.
Product Images

25(OH) Vitamin D Standard Curve: ELISA plate was coated with
100 ng capture antibody, Clone RMH04 at concentration of 1 µg/mL. A serial
dilution from 150 ng/mL was performed using 25(OH) vitamin D.
Biotin-labeled 25(OH) Vitamin D antibody, Clone RM428 was
used as a detection antibody.

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