Aconitase Activity Assay Kit (Colorimetric)
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 靶点名称:
- 偶联标记说明:
- 灵敏度:
- 检测范围:
- 样品类型:
- 样本量:
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Brand:
CAT.NO. : ARG82129
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Product Details
概述
| 产品描述 | ARG82129 Aconitase Activity Assay Kit (Colorimetric) can be used to measure Aconitase activity in cell lysate, tissue homogenate, serum. |
|---|---|
| 反应物种 | Hu, Ms, Rat, All |
| 应用 | FuncSt |
| 靶点名称 | Aconitase |
| 偶联标记说明 | Read at 565 nm |
| 灵敏度 | 0.5 U/L |
| 检测范围 | 0.5 - 100 U/L |
| 样品类型 | cell lysate, tissue homogenate, serum |
| 样本量 | 20 µl |
| 別名 | HEL60; Aconitase; EC 4.2.1.3; Cytoplasmic aconitate hydratase; Ferritin repressor protein; IRP1; Iron regulatory protein 1; IRE-BP 1; Iron-responsive element-binding protein 1; Citrate hydro-lyase; ACONS; IREB1; IREBP; IREBP1 |
应用说明
| 应用说明 | Please note that this kit does not include a microplate. |
|---|---|
| 检测时间 | 30 min |
属性
| 形式 | Liquid |
|---|---|
| 存放说明 | Store the kit at -20°C. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ACO1 |
| 全名 | aconitase 1, soluble |
| 背景介绍 | The protein encoded by this gene is a bifunctional, cytosolic protein that functions as an essential enzyme in the TCA cycle and interacts with mRNA to control the levels of iron inside cells. When cellular iron levels are high, this protein binds to a 4Fe-4S cluster and functions as an aconitase. Aconitases are iron-sulfur proteins that function to catalyze the conversion of citrate to isocitrate. When cellular iron levels are low, the protein binds to iron-responsive elements (IREs), which are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. When the protein binds to IRE, it results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degraded transferrin receptor mRNA. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alternative splicing results in multiple transcript variants [provided by RefSeq, Jan 2014] |
| 生物功能 | Iron sensor. Binds a 4Fe-4S cluster and functions as aconitase when cellular iron levels are high. Functions as mRNA binding protein that regulates uptake, sequestration and utilization of iron when cellular iron levels are low. Binds to iron-responsive elements (IRES) in target mRNA species when iron levels are low. Binding of a 4Fe-4S cluster precludes RNA binding. Catalyzes the isomerization of citrate to isocitrate via cis-aconitate. [UniProt] |
| 细胞定位 | Cytoplasm. [UniProt] |
| 产品亮点 | Related products: Aconitase antibodies; Aconitase ELISA Kits; Related news: TCA intermediate fumarate promotes mitobiogenesis |
检测图片 (1)
ARG82129 Aconitase Activity Assay Kit (Colorimetric) enzyme kinetics graph
Kinetics of 0 and 50 U/L Aconitase reaction, using ARG82129 Aconitase Activity Assay Kit (Colorimetric).
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