Alkaline Phosphatase Activity Assay Kit (Colorimetric)

Key features and details

  • 产品描述: ARG81239 Alkaline Phosphatase Activity Assay Kit (Colorimetric) can be used to measure Alkaline Phosphatase activity in Human or Mouse cells.
  • 反应物种: Hu, Ms
  • 应用: FuncSt
  • 靶点名称: Alkaline Phosphatase Activity
  • 偶联标记: Un-conjugated
  • 偶联标记说明: Read at 405 nm
  • 样品类型: Cultured cells (cell lysate)
  • 标准范围: p - nitrophenol: 0.3 - 19.2 nmol
  • 別名: TNSALP; HOPS; EC 3.1.3.1; Alkaline phosphatase, tissue-nonspecific isozyme; TNAP; Alkaline phosphatase liver/bone/kidney isozyme; APTNAP; AP-TNAP
  • Brand:
CAT.NO. : ARG81239
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Product Details
内含物
货号内含物名称包装
ARG81239-01Alkaline Phosphatase Activity Substrate5 ml
ARG81239-02Standard (p-Nitrophenol)1 ml
ARG81239-03Cell Lysis Buffer20 ml
ARG81239-04Stop Solution (10X)10 ml
概述
产品描述ARG81239 Alkaline Phosphatase Activity Assay Kit (Colorimetric) can be used to measure Alkaline Phosphatase activity in Human or Mouse cells.
反应物种Hu, Ms
应用FuncSt
靶点名称Alkaline Phosphatase Activity
偶联标记Un-conjugated
偶联标记说明Read at 405 nm
样品类型Cultured cells (cell lysate)
标准范围p - nitrophenol: 0.3 - 19.2 nmol
別名TNSALP; HOPS; EC 3.1.3.1; Alkaline phosphatase, tissue-nonspecific isozyme; TNAP; Alkaline phosphatase liver/bone/kidney isozyme; APTNAP; AP-TNAP
应用说明
检测时间~ 1 hour
属性
形式Liquid
存放说明Store the kit at 2-8°C. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 11647 Mouse ALPL

GeneID: 249 Human ALPL

Swiss-port # P05186 Human Alkaline phosphatase, tissue-nonspecific isozyme

Swiss-port # P09242 Mouse Alkaline phosphatase, tissue-nonspecific isozyme

基因名称ALPL
全名alkaline phosphatase, liver/bone/kidney
背景介绍There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization; however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2010]
生物功能This isozyme may play a role in skeletal mineralization. [UniProt]
翻译后修饰N-glycosylated.
检测图片 (1)
  • ARG81239 Alkaline Phosphatase Activity Assay Kit (Colorimetric) standard curve image

    ARG81239 Alkaline Phosphatase Activity Assay Kit (Colorimetric) results of a typical standard run with optical density reading at 405 nm.

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