ALT / Alanine Transaminase Assay Kit
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 靶点名称:
- 偶联标记说明:
- 灵敏度:
- 检测范围:
- 样品类型:
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Brand:
CAT.NO. : ARG81298
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Product Details
概述
| 产品描述 | ARG81298 ALT / Alanine Transaminase Assay Kit is a detection kit for the quantification of ALT / Alanine Transaminase in serum and plasma. |
|---|---|
| 反应物种 | Hu, Ms, Rat, Mamm |
| 应用 | FuncSt |
| 特异性 | Alanine Transaminase (ALT) facilitates the conversion of alanine and α-ketoglutarate to pyruvate and glutamate. Then pyruvate and NADH are converted to lactate and NAD by lactate dehydrogenase (LDH).Therefore, the decrease in NADH absorbance at 340 nm is proportionate to ALT activity. |
| 靶点名称 | ALT / Alanine Transaminase |
| 偶联标记说明 | Read at 340 nm. |
| 灵敏度 | 2 U/l |
| 检测范围 | 2 - 100 U/l |
| 样品类型 | Serum and plasma. |
| 样本量 | 20 µl |
| 別名 | Glutamate pyruvate transaminase 1; AAT1; GPT1; GPT 1; Glutamic--alanine transaminase 1; Glutamic--pyruvic transaminase 1; Alanine aminotransferase 1; ALT1; EC 2.6.1.2 |
应用说明
| 应用说明 | Please note that this kit does not include a microplate. |
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| 检测时间 | 10 min |
属性
| 形式 | Liquid |
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| 存放说明 | Store the kit at -20°C. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | GPT |
| 全名 | glutamic-pyruvate transaminase (alanine aminotransferase) |
| 背景介绍 | This gene encodes cytosolic alanine aminotransaminase 1 (ALT1); also known as glutamate-pyruvate transaminase 1. This enzyme catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.[provided by RefSeq, Nov 2009] |
| 生物功能 | Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles (By similarity). [UniProt] |
检测图片 (1)
ARG81298 ALT / Alanine Transaminase Assay Kit functional study image
Functional study: ARG81298 ALT / Alanine Transaminase Assay Kit results of a typical example of ALT activity reading at 340 nm.
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