anti-12 Lipoxygenase antibody
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
- 抗原:
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Brand:
Product Details
| 产品描述 | Rabbit Polyclonal antibody recognizes 12 Lipoxygenase |
|---|---|
| 反应物种 | Hu, Ms, Rb |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | 12 Lipoxygenase |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide corresponding to aa. 618-650 (C-terminus) of Human 12 Lipoxygenase. |
| 偶联标记 | Un-conjugated |
| 別名 | EC 1.13.11.31; Platelet-type lipoxygenase 12; 12S-LOX; 12-LOX; 12S-lipoxygenase; Arachidonate 12-lipoxygenase, 12S-type; EC 3.3.2.-; LOG12; Lipoxin synthase 12-LO |
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| 阳性对照 | K562 |
| 形式 | Liquid |
|---|---|
| 纯化 | This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide |
| 抗菌剂 | 0.09% (W/V) Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
| 数据库连接 | Swiss-port # P18054 Human Arachidonate 12-lipoxygenase, 12S-type Swiss-port # P39655 Mouse Arachidonate 12-lipoxygenase, 12S-type |
|---|---|
| 基因名称 | ALOX12 |
| 全名 | arachidonate 12-lipoxygenase |
| 生物功能 | Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Mainly converts arachidonic acid to (12S)-hydroperoxyeicosatetraenoic acid/(12S)-HPETE but can also metabolize linoleic acid. Has a dual activity since it also converts leukotriene A4/LTA4 into both the bioactive lipoxin A4/LXA4 and lipoxin B4/LXB4. Through the production of specific bioactive lipids like (12S)-HPETE it regulates different biological processes including platelet activation. It also probably positively regulates angiogenesis through regulation of the expression of the vascular endothelial growth factor. Plays a role in apoptotic process, promoting the survival of vascular smooth muscle cells for instance. May also play a role in the control of cell migration and proliferation. [UniProt] |
| 细胞定位 | Cytoplasm, cytosol. Membrane. Note=Membrane association is stimulated by EGF |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody |
| 预测分子量 | 76 kDa |
ARG55210 anti-12 Lipoxygenase antibody ICC/IF image
Immunofluorescence: A549 cells stained with ARG55210 anti-12 Lipoxygenase antibody (green) at 1:100 dilution. Cytoplasmic actin was counterstained with Dylight Fluor® 554 conjugated Phalloidin (red).
ARG55210 anti-12 Lipoxygenase antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human skin tissue stained with ARG55210 anti-12 Lipoxygenase antibody.
ARG55210 anti-12 Lipoxygenase antibody WB image
Western blot: 35 µg of K562 cell lysate stained with ARG55210 anti-12 Lipoxygenase antibody.
ARG55210 anti-12 Lipoxygenase antibody FACS image
Flow Cytometry: K562 cells stained with ARG55210 anti-12 Lipoxygenase antibody (bottom histogram) or without primary antibody control (top histogram), followed by incubation with FITC labelled secondary antibody.
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