anti-14-3-3 gamma antibody [4B9]

Key features and details

  • 产品描述: Mouse Monoclonal antibody [4B9] recognizes 14-3-3 gamma
  • 反应物种: Hu
  • 应用: ICC/IF, WB
  • 宿主: Mouse
  • 克隆: Monoclonal
  • 克隆号: 4B9
  • 同位型: IgG1, kappa
  • 靶点名称: 14-3-3 gamma
  • 抗原物种: Human
  • Brand:
CAT.NO. : ARG57137
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Product Details
概述
产品描述Mouse Monoclonal antibody [4B9] recognizes 14-3-3 gamma
反应物种Hu
应用ICC/IF, WB
宿主Mouse
克隆Monoclonal
克隆号4B9
同位型IgG1, kappa
靶点名称14-3-3 gamma
抗原物种Human
抗原Recombinant fragment around aa. 1-247 of Human 14-3-3 gamma
偶联标记Un-conjugated
別名14-3-3 protein gamma; PPP1R170; 14-3-3GAMMA; KCIP-1; Protein kinase C inhibitor protein 1
应用说明
应用建议
应用推荐稀释比
ICC/IFAssay-dependent
WB1:1000
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Purification with Protein A.
缓冲液PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol.
抗菌剂0.02% Sodium azide
稳定剂10% Glycerol
浓度1 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 7532 Human YWHAG

Swiss-port # P61981 Human 14-3-3 protein gamma

基因名称YWHAG
全名tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma
背景介绍This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways. [provided by RefSeq, Jul 2008]
生物功能Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. [UniProt]
预测分子量28 kDa
翻译后修饰Phosphorylated by various PKC isozymes.
检测图片 (4)
  • ARG57137 anti-14-3-3 gamma antibody [4B9] ICC/IF image

    Immunofluorescence: HeLa cells line stained with ARG57137 anti-14-3-3 gamma antibody [4B9] at 1:100 (Green).

    DAPI (Blue) for nucleus staining.

  • ARG57137 anti-14-3-3 gamma antibody [4B9] WB image

    Western blot: 40 µg of HeLa cell and Mouse brain lysates stained with ARG57137 anti-14-3-3 gamma antibody [4B9] at 1:1000.

  • ARG57137 anti-14-3-3 gamma antibody [4B9] WB image

    Western blot: 50 ng of Human 1) YWHAZ, 2) YWHAB, 3) YWHAE, 4) YWHAH, 5) YWHAG, 6) SFN, and 7) YWHAQ recombinant proteins stained with ARG57137 anti-14-3-3 gamma antibody [4B9] at 1:1000.

  • ARG57137 anti-14-3-3 gamma antibody [4B9] WB image

    Western blot: 40 µg of 1) 293T, 2) A431, 3) K562, and 4) NIH3T3 cell lysates stained with ARG57137 anti-14-3-3 gamma antibody [4B9] at 1:1000.

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