anti-14-3-3 tau / YWHAQ antibody [1A1]

CAT.NO. : ARG57092

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Size:

50 μl

Product Details

概述

产品描述	Mouse Monoclonal antibody [1A1] recognizes 14-3-3 tau / YWHAQ
反应物种	Hu
应用	ICC/IF, WB
宿主	Mouse
克隆	Monoclonal
克隆号	1A1
同位型	IgG1, kappa
靶点名称	14-3-3 tau / YWHAQ
抗原物种	Human
抗原	Recombinant fragment around aa. 1-245 of Human 14-3-3 tau / YWHAQ
偶联标记	Un-conjugated
別名	14-3-3; Protein HS1; 1C5; HS1; 14-3-3 protein theta; 14-3-3 protein tau; 14-3-3 protein T-cell

应用说明

应用建议

应用	推荐稀释比
ICC/IF	Assay-dependent
WB	1:1000 - 1:2000

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Purification with Protein G.
缓冲液	PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol.
抗菌剂	0.02% Sodium azide
稳定剂	10% Glycerol
浓度	1 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 10971 Human YWHAQ Swiss-port # P27348 Human 14-3-3 protein theta
基因名称	YWHAQ
全名	tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta
背景介绍	This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease. [provided by RefSeq, Jul 2008]
生物功能	Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1. [UniProt]
预测分子量	28 kDa
翻译后修饰	Ser-232 is probably phosphorylated by CK1.

检测图片 (4)

ARG57092 anti-14-3-3 tau / YWHAQ antibody [1A1] ICC/IF image
Immunofluorescence: HeLa cells line stained with ARG57092 anti-14-3-3 tau / YWHAQ antibody [1A1] at 1:100 (Green).
DAPI (Blue) for nucleus staining.
ARG57092 anti-14-3-3 tau / YWHAQ antibody [1A1] WB image
Western blot: 20 µg of Jurkat cell lysate stained with ARG57092 anti-14-3-3 tau / YWHAQ antibody [1A1] at 1:1000.
ARG57092 anti-14-3-3 tau / YWHAQ antibody [1A1] WB image
Western blot: 50 ng of Human 1) YWHAZ, 2) YWHAB, 3) YWHAE, 4) YWHAH, 5) YWHAG, 6) SFN, and 7) YWHAQ recombinant proteins stained with ARG57092 anti-14-3-3 tau / YWHAQ antibody [1A1] at 1:1000.
ARG57092 anti-14-3-3 tau / YWHAQ antibody [1A1] WB image
Western blot: 40 µg of 1) MDA-MB-21, 2) HeLa, 3) A431, 4) NIH3T3, 5) 293T, and 6) HepG2 cell lysates stained with ARG57092 anti-14-3-3 tau / YWHAQ antibody [1A1] at 1:1000.

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