anti-4-Hydroxy-2-hexenal / 4-HHE antibody [6F10]
![anti-4-Hydroxy-2-hexenal / 4-HHE antibody [6F10]](/upload/image/products/ARG22618_WB_1_210_205.jpg)
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![anti-4-Hydroxy-2-hexenal / 4-HHE antibody [6F10]](/upload/image/products/ARG22618_WB_1.jpg)
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 宿主:
- 克隆:
- 克隆号:
- 同位型:
- 靶点名称:
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Brand:
Product Details
| 产品描述 | Mouse Monoclonal antibody [6F10] recognizes 4-Hydroxy-2-hexenal / 4-HHE |
|---|---|
| 反应物种 | Other |
| 应用 | ICC/IF, WB |
| 特异性 | Specific for 4-Hydroxy-2-hexenal (4-HHE) and 4-Hydroxynonenal (4-HNE) modified proteins. Does not detect free 4-Hydroxy-2-hexenal (4-HHE) or 4-Hydroxynonenal (4-HNE). Does not cross-react with Acrole. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 6F10 |
| 同位型 | IgG2b |
| 靶点名称 | 4-Hydroxy-2-hexenal / 4-HHE |
| 抗原物种 | Others |
| 抗原 | Synthetic 4-Hydroxy-2-hexenal modified KLH. |
| 偶联标记 | Un-conjugated |
| 应用建议 |
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|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS (pH 7.4), 0.9% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.9% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
ARG22618 anti-4-Hydroxy-2-hexenal / 4-HHE antibody [6F10] ICC/IF image
Immunofluorescence: 5% Formaldehyde fixed (5 min) HEK293 cells stained with ARG22618 anti-4-Hydroxy-2-hexenal / 4-HHE antibody [6F10] at 1:400 for 30 - 60 min at RT. Secondary Antibody: Goat anti-Mouse (Alexa Fluor 488) at 1:1500 for 30 - 60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain. (A,C,E,G) Untreated. (B,D,F,H) Cells cultured overnight with 50 µM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Primary antibody. (G,H) Composite.
ARG22618 anti-4-Hydroxy-2-hexenal / 4-HHE antibody [6F10] WB image
Western blot: BSA (0.5 µg), 4-hydroxynonenal-BSA (0.5 µg), 4-hydroxynonenal-BSA (2.0 µg), 4-hydroxy-2-hexenal (0.5 µg), and 4-hydroxy-2-hexenal (2.0 µg) stained with ARG22618 anti-4-Hydroxy-2-hexenal / 4-HHE antibody [6F10] at 1:1000 for 2 hours at RT.
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