anti-4 Hydroxynonenal antibody [12F7]
![anti-4 Hydroxynonenal antibody [12F7]](/upload/image/products/ARG23717_WB_1_210_205.jpg)
![anti-4 Hydroxynonenal antibody [12F7]](/upload/image/products/ARG23717.png)
![anti-4 Hydroxynonenal antibody [12F7]](/upload/image/products/ARG23717_IF_HEK293.jpg)
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Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 宿主:
- 克隆:
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- 同位型:
- 靶点名称:
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Brand:
Product Details
| 产品描述 | Mouse Monoclonal antibody [12F7] recognizes 4 Hydroxynonenal |
|---|---|
| 反应物种 | Other |
| 应用 | ELISA, ICC/IF, IHC-P, WB |
| 特异性 | Specific for 4-Hydroxynonenal (4-HNE) modified proteins. Does not detect free 4-Hydroxynonenal. Does not cross-react with 4-Hydroxy-2-hexenal, Acrolein, Crotonaldehyde, Hexanoyl Lysine, Malondialdehyde, or Methylglyoxal modified proteins. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 12F7 |
| 同位型 | IgG1 |
| 靶点名称 | 4 Hydroxynonenal |
| 抗原物种 | Others |
| 抗原 | Synthetic 4-Hydroxynonenal modified KLH. |
| 偶联标记 | Un-conjugated |
| 別名 | 4-HNE |
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS (pH 7.4), 0.09% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.09% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
| 产品亮点 | Related Antibody Duos and Panels: ARG30337 Ferroptosis / Oxytosis Antibody Panel Related products: Hydroxynonenal antibodies; Hydroxynonenal ELISA Kits; Hydroxynonenal Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: Ferroptosis/Oxytosis Antibody Panel is launched Pericytes, new therapeutic target for Alzheimer's disease? Therapeutic strategies against PDAC |
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| 研究领域 | Ferroptosis/Oxytosis Study antibody; Lipid peroxidation Marker antibody |
ARG23717 anti-4 Hydroxynonenal antibody [12F7] IHC-P image
Immunohistochemistry: Mouse testis stained with ARG23717 anti-4 Hydroxynonenal antibody [12F7] at 1:50 dilution.
From Lipeng Li et al. FDX1 regulates leydig cell ferroptosis mediates PM2.5-induced testicular dysfunction of mice (2023), doi: 10.1016/j.ecoenv.2023.115309, Fig. 4A1.
ARG23717 anti-4 Hydroxynonenal antibody [12F7] ICC/IF image
Immunofluorescence: HEK293 cells fixed with 5% Formaldehyde (5 min). Cells were stained with ARG23717 anti-4 Hydroxynonenal antibody [12F7] at 1:50 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G): Untreated. (B,D,F,H): Cells cultured overnight with 50 µM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Primary antibodies. (G,H) Composite.
ARG23717 anti-4 Hydroxynonenal antibody [12F7] WB image
Western blot: 0.5 µg of BSA, 0.5 µg of 4-hydroxyl nonenal-BSA, 2.0 µg of 4-hydroxy nonenal-BSA, 0.5 µg of 4-hydroxy-2-hexenal and 2.0 µg of 4-hydroxy-2-hexenal stained with ARG23717 anti-4 Hydroxynonenal antibody [12F7] at 1:1000 for 2 hours at RT.
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