anti-4E-T / EIF4ENIF1 antibody

Key features and details

  • 产品描述: Goat Polyclonal antibody recognizes 4E-T / EIF4ENIF1
  • 反应物种: Hu
  • 预测物种: Ms, Rat, Cow, Dog, Pig
  • 应用: IHC-P, WB
  • 特异性: This antibody is expected to recognise isoform 1 (NP_062817.2) and isoform b (NP_001157974.1). Reported variants represent identical protein (NP_062817.2; NP_001157973.1).
  • 宿主: Goat
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: 4E-T / EIF4ENIF1
  • Brand:
CAT.NO. : ARG63405
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Product Details
概述
产品描述Goat Polyclonal antibody recognizes 4E-T / EIF4ENIF1
反应物种Hu
预测物种Ms, Rat, Cow, Dog, Pig
应用IHC-P, WB
特异性This antibody is expected to recognise isoform 1 (NP_062817.2) and isoform b (NP_001157974.1). Reported variants represent identical protein (NP_062817.2; NP_001157973.1).
宿主Goat
克隆Polyclonal
同位型IgG
靶点名称4E-T / EIF4ENIF1
抗原物种Human
抗原C-AKVISVDELEYRQ
偶联标记Un-conjugated
別名4E-T; Eukaryotic translation initiation factor 4E nuclear import factor 1; Eukaryotic translation initiation factor 4E transporter; eIF4E transporter; Clast4
应用说明
应用建议
应用推荐稀释比
IHC-P5 - 10 µg/ml
WB0.25 - 0.5 µg/ml
应用说明IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
缓冲液Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
抗菌剂0.02% Sodium azide
稳定剂0.5% BSA
浓度0.5 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 56478 Human EIF4ENIF1

Swiss-port # Q9NRA8 Human Eukaryotic translation initiation factor 4E transporter

背景介绍The protein encoded by this gene is a nucleocytoplasmic shuttle protein for the translation initiation factor eIF4E. This shuttle protein interacts with the importin alpha-beta complex to mediate nuclear import of eIF4E. It is predominantly cytoplasmic; its own nuclear import is regulated by a nuclear localization signal and nuclear export signals. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009]
研究领域Gene Regulation antibody
预测分子量108 kDa
检测图片 (2)
  • ARG63405 anti-4E-T / EIF4ENIF1 antibody WB image

    Western Blot: 293 lysate (RIPA buffer, 35 µg total protein per lane) stained with ARG63405 anti-4E-T / EIF4ENIF1 antibody at 0.25 µg/ml dilution.

  • ARG63405 anti-4E-T / EIF4ENIF1 antibody IHC-P image

    Immunohistochemistry: paraffin embedded Human Pancreas. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63405 anti-4E-T / EIF4ENIF1 antibody at 5 µg/ml dilution followed by AP-staining.

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