anti-5-bromodeoxyuridine / BrdU antibody [Bu20a] (PE)
![anti-5-bromodeoxyuridine / BrdU antibody [Bu20a] (PE)](/upload/image/products/ARG54229_FACS_1_201117_210_205.jpg)
![anti-5-bromodeoxyuridine / BrdU antibody [Bu20a] (PE)](/upload/image/products/ARG54229_FACS_1_201117.jpg)
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CAT.NO. : ARG54229
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Product Details
概述
| 产品描述 | PE-conjugated Mouse Monoclonal antibody [Bu20a] recognizes 5-bromodeoxyuridine / BrdU |
|---|---|
| 反应物种 | Other |
| 应用 | FACS |
| 特异性 | The clone Bu20a reacts specifically with BrdU incorporated into DNA during S-phase of a cell cycle. It is useful for detecting proliferating cells by flow cytometry or immunohistochemistry staining. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | Bu20a |
| 同位型 | IgG1 |
| 靶点名称 | 5-bromodeoxyuridine / BrdU |
| 抗原 | Bromodeoxyuridine conjugated to BSA |
| 偶联标记 | PE |
应用说明
| 应用建议 |
| ||||
|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化说明 | The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary. |
| 缓冲液 | PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA |
| 抗菌剂 | 15 mM Sodium azide |
| 稳定剂 | 0.2% (w/v) high-grade protease free BSA |
| 存放说明 | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 背景介绍 | Bromodexyuridine (BrdU) is a thymidine analog which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates, tissue sections or suspensions using an antibody specific for the BrdU. |
|---|---|
| 研究领域 | Cell Biology and Cellular Response antibody; Controls and Markers antibody; Gene Regulation antibody; Neuroscience antibody |
检测图片 (1)
ARG54229 anti-5-bromodeoxyuridine / BrdU antibody [Bu20a] (PE) FACS image
Flow Cytometry: BrdU incorporated into the K562 cells. Cells were stained with ARG54229 anti-5-bromodeoxyuridine / BrdU antibody [Bu20a] (PE).
参考文献
克隆号文献
Presentation of fibronectin fragments using affinity protein interactions for enhanced retention and function.
Double immunocytochemical labeling of cell and tissue samples with monoclonal anti-bromodeoxyuridine.
ICC/IF / Human, Mouse
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