anti-5-bromodeoxyuridine / BrdU antibody [MoBu-1]

Key features and details

  • 产品描述: Mouse Monoclonal antibody [MoBu-1] recognizes 5-bromodeoxyuridine / BrdU
  • 反应物种: Other
  • 应用: FACS, ICC/IF, IHC-Fr, IHC-P
  • 特异性: The clone MoBu-1 reacts specifically with BrdU incorporated into DNA during S-phase of a cell cycle. MoBu-1 is also useful for detecting proliferating cells by flow cytometry or immunofluorescence staining. It reacts also specifically with 5-bromouridine (BrU). The clone does not cross-react to EdU (PMID: 23272138).
  • 宿主: Mouse
  • 克隆: Monoclonal
  • 克隆号: MoBu-1
  • 同位型: IgG1
  • 靶点名称: 5-bromodeoxyuridine / BrdU
  • Brand:
CAT.NO. : ARG63121
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Product Details
概述
产品描述Mouse Monoclonal antibody [MoBu-1] recognizes 5-bromodeoxyuridine / BrdU
反应物种Other
应用FACS, ICC/IF, IHC-Fr, IHC-P
特异性The clone MoBu-1 reacts specifically with BrdU incorporated into DNA during S-phase of a cell cycle. MoBu-1 is also useful for detecting proliferating cells by flow cytometry or immunofluorescence staining. It reacts also specifically with 5-bromouridine (BrU). The clone does not cross-react to EdU (PMID: 23272138).
宿主Mouse
克隆Monoclonal
克隆号MoBu-1
同位型IgG1
靶点名称5-bromodeoxyuridine / BrdU
抗原5-bromodeoxyuridine conjugated with hemocyanine.
偶联标记Un-conjugated
应用说明
应用建议
应用推荐稀释比
FACS1 - 2 µg/ml
ICC/IF2 µg/ml
IHC-FrAssay-dependent
IHC-PAssay-dependent
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Purified from ascites by protein-A affinity chromatography.
纯度> 95% (by SDS-PAGE)
缓冲液PBS (pH 7.4) and 15 mM Sodium azide
抗菌剂15 mM Sodium azide
浓度1 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
背景介绍Bromodexyuridine (BrdU) is a thymidine analog which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates, tissue sections or suspensions using an antibody specific for the BrdU.
产品亮点Related products:
BrdU antibodies; BrdU Duos / Panels; Anti-Mouse IgG secondary antibodies;
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研究领域Cell Biology and Cellular Response antibody; Controls and Markers antibody; Gene Regulation antibody; Neuroscience antibody
检测图片 (2)
  • ARG63121 anti-5-bromodeoxyuridine / BrdU antibody [MoBu-1] FACS image

    Flow Cytometry: 5-bromodeoxyuridin (BrdU) incorporated CEM cells stained with ARG63121 anti-5-bromodeoxyuridine / BrdU antibody [MoBu-1], followed by incubation with FITC-labelled secondary antibody.

  • ARG63121 anti-5-bromodeoxyuridine / BrdU antibody [MoBu-1] IHC-P image

    Immunohistochemistry: Bromodeoxyuridine-labeled cells (chick embryo; paraffin-embedded sections) stained with ARG63121 anti-5-bromodeoxyuridine / BrdU antibody [MoBu-1].

参考文献

Vascular Endothelial Cell-derived Exosomes Protect Neural Stem Cells Against Ischemia/reperfusion Injury.

IHC-Fr / Other

Zhou Shaoting et al.
Neuroscience.,  (2020)

publication_link

 

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Activation of AMPK pathway compromises Rab11 downregulation-mediated inhibition of Schwann cell proliferation in a Glut1 and Glut3-dependent manner.

ICC/IF / Other

Li Fan et al.
Neurosci Lett.,  (2020)

publication_link

 

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