anti-5-HT3A Receptor antibody

Key features and details

  • 产品描述: Goat Polyclonal antibody recognizes 5-HT3A Receptor
  • 反应物种: Hu, Rat
  • 应用: ICC/IF, IHC-P, WB
  • 特异性: This antibody is expected to recognise all three reported isoforms (NP_998786.2; NP_000860.2; NP_001155244.1).
  • 宿主: Goat
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: 5-HT3A Receptor
  • 抗原物种: Human
  • Brand:
CAT.NO. : ARG64018
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Product Details
概述
产品描述Goat Polyclonal antibody recognizes 5-HT3A Receptor
反应物种Hu, Rat
应用ICC/IF, IHC-P, WB
特异性This antibody is expected to recognise all three reported isoforms (NP_998786.2; NP_000860.2; NP_001155244.1).
宿主Goat
克隆Polyclonal
同位型IgG
靶点名称5-HT3A Receptor
抗原物种Human
抗原C-GPQDFEKSPRDR
偶联标记Un-conjugated
別名5-HT-3; 5-hydroxytryptamine receptor 3; Serotonin-gated ion channel receptor; 5-HT3A; 5-HT3-A; HTR3; 5HT3R; Serotonin receptor 3A; 5-hydroxytryptamine receptor 3A; 5-HT3R
应用说明
应用建议
应用推荐稀释比
ICC/IFAssay - dependent
IHC-P3 - 5 µg/ml
WB0.3 - 1 µg/ml
应用说明WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
缓冲液Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
抗菌剂0.02% Sodium azide
稳定剂0.5% BSA
浓度0.5 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 3359 Human HTR3A

GeneID: 79246 Rat HTR3A

Swiss-port # P35563 Rat 5-hydroxytryptamine receptor 3A

Swiss-port # P46098 Human 5-hydroxytryptamine receptor 3A

背景介绍The product of this gene belongs to the ligand-gated ion channel receptor superfamily. This gene encodes subunit A of the type 3 receptor for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor causes fast, depolarizing responses in neurons after activation. It appears that the heteromeric combination of A and B subunits is necessary to provide the full functional features of this receptor, since either subunit alone results in receptors with very low conductance and response amplitude. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
研究领域Metabolism antibody; Neuroscience antibody
预测分子量55 kDa
检测图片 (2)
  • ARG64018 anti-5-HT3A Receptor antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human small intestine tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64018 anti-5-HT3A Receptor antibody at 3.75 µg/ml dilution followed by AP-staining.

  • ARG64018 anti-5-HT3A Receptor antibody WB image

    Western blot: 35 µg of Human Colon lysate (in RIPA buffer) stained with ARG64018 anti-5-HT3A Receptor antibody at 0.3 µg/ml dilution and incubated at RT for 1 hour.

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