anti-5 Lipoxygenase phospho (Ser523) antibody
Key features and details
- 产品描述:
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- 预测物种:
- 应用:
- 宿主:
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- 靶点名称:
- 抗原物种:
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Brand:
CAT.NO. : ARG52216
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Product Details
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes 5 Lipoxygenase phospho (Ser523) |
|---|---|
| 反应物种 | Hu, Rat |
| 预测物种 | NHuPrm |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | 5 Lipoxygenase |
| 抗原物种 | Human |
| 抗原 | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser523 conjugated to KLH |
| 偶联标记 | Un-conjugated |
| 別名 | 5-lipoxygenase; Arachidonate 5-lipoxygenase; LOG5; 5-LO; EC 1.13.11.34; 5LPG; 5-LOX |
应用说明
| 应用建议 |
| ||||
|---|---|---|---|---|---|
| 应用说明 | Specific for the ~80k 5-LO phosphorylated at Ser523 in Western blots. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity Purified |
| 缓冲液 | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| 稳定剂 | 0.1 mg/ml BSA, 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ALOX5 |
| 全名 | arachidonate 5-lipoxygenase |
| 背景介绍 | The enzyme 5-lipoxygenase (5-LO) plays a key role in regulating the production of leukotrienes (LTs) (Funk, 2001). Overproduction of LTs contributes to several diseases, most notably chronic inflammatory diseases, including asthma (Drazen et al., 1994), fibrosis (Wilborn et al., 1996) and atherosclerosis (Dwyer et al., 2004). Recent work has demonstrated that the activity of 5-LO is regulated by PKA phosphorylation of serine-523 in 5-LO (Luo et al., 2004). Under normal conditions, this phosphorylation may be important in limiting inflammation. Abnormal signaling through cAMP and PKA, then, could contribute to a variety of diseases, including those characterized by chronic inflammation. The phospho-specific antibody to Ser523 on 5-LO is thus likely to provide a valuable tool for studies of the role of 5-LO regulation in diseases such as asthma, fibrosis and atherosclerosis. |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody |
| 预测分子量 | 78 kDa |
| 翻译后修饰 | Serine phosphorylation by MAPKAPK2 is stimulated by arachidonic acid. Phosphorylation on Ser-523 by PKA has an inhibitory effect. Phosphorylation on Ser-272 prevents export from the nucleus. |
检测图片 (1)
ARG52216 anti-5 Lipoxygenase phospho (Ser523) antibody WB image
Western blot: Rat hippocampal lysate showing phospho-specific immunolabeling of the ~80k doublet of 5-LO phosphorylated at Ser 523 stained with ARG52216 anti-5 Lipoxygenase phospho (Ser523) antibody.
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