Anti-5-methylcytosine (5-mC) Rabbit Monoclonal Antibody; [Clone RM231]; 5′-mC; 31-1110-00
![Anti-5-methylcytosine (5-mC) Rabbit Monoclonal Antibody; [Clone RM231]; 5′-mC; 31-1110-00](/upload/image/products/Fig3_mC_ICC_edit.png)
![Anti-5-methylcytosine (5-mC) Rabbit Monoclonal Antibody; [Clone RM231]; 5′-mC; 31-1110-00](/upload/image/products/Fig1_5mC_ELISA.png)
![Anti-5-methylcytosine (5-mC) Rabbit Monoclonal Antibody; [Clone RM231]; 5′-mC; 31-1110-00](/upload/image/products/Fig4_mC_DirectELISA_E.png)
![Anti-5-methylcytosine (5-mC) Rabbit Monoclonal Antibody; [Clone RM231]; 5′-mC; 31-1110-00](/upload/image/products/Fig5_mC_MeDIP_e.png)
![Anti-5-methylcytosine (5-mC) Rabbit Monoclonal Antibody; [Clone RM231]; 5′-mC; 31-1110-00](/upload/image/products/Fig2_5mC_dotblot.png)
Key features and details
- Uniprot:
- Gene ID:
- Host Species:
- Species Reactivity:
- Concentration:
-
Brand:
Product Details
| Product Name | Anti-5-methylcytosine (5-mC) Rabbit Monoclonal Antibody, Clone RM231 |
| Product Description | Rabbit monoclonal to 5-methylcytosine (5-mC) Rabbit Monoclonal Antibody, 5'-mC |
| Catalog No. | 31-1110-00 |
| Clone Name | RM231 |
| Product Data Sheet | Anti-5-methylcytosine (5-mC) Rabbit Monoclonal Antibody Data Sheet RM231 |
| Specificity | RM231 reacts to 5-methylcytosine in both single-stranded and double-stranded DNA. No cross reactivity with non-methylated cytosine and hydroxymethylcytosine in DNA. |
| Immunogen | BSA-conjugated 5-methylcytosine. |
| Application | Methylated DNA immunoprecipitation (MeDIP) ELISA Dot Blot (DB) Immunocytochemistry (ICC) Flow Cytometry (FC) |
| Species Reactivity | All |
| Conjugate | None |
| Intended Use | Research Use Only |
| Related Product | 5-Methylcytosine (5-mC) DNA Matched Antibody Pair |
| . | |
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt. |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Size | 50 µg |
| Concentration | 1.0 mg/mL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | MeDIP: 0.2 µg/mL - 2 µg/mL; ELISA: 0.1 µg/mL - 1 ug/mL; Dot Blot: 0.5 µg/mL – 2 µg/mL; ICC: 0.5 µg/mL - 2 µg/mL; FC: 0.5 ug/mL - 2 ug/mL. |
| References for 5-mC antibody (RM231) | Logsdon GA et al The structure, function and evolution of a complete human chromosome 8. Nature. (2021), 10.1038/s41586-021-03420-7. IF; Human. Read more (PubMed: 33828295) Kojima N et al. Immobilization of DNA with nitrogen mustard-biotin conjugate for global epigenetic analysis. Anal Chim Acta. (2018), 10.1016/j.aca.2018.09.008. ELISA; Human. Read more (PubMed: 30392657) |
| Keywords | |
| . | |
 | ELISA of single stranded DNA using rabbit monoclonal anti-5-mC (clone RM231) antibody. The plate was coated with streptavidin and then biotinylated single stranded unmethylated DNA, 5-Methylcytosine (5-mC) DNA, and 5-Hydroxymethylcytosine (5-hmC) DNA. A serial dilution of RM231 was used as the primary antibody, and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody. |
 | Dot blot of double stranded DNA using rabbit monoclonal anti-5-mC (clone RM231) antibody. The membrane was pre-spotted with 50, 5, and 0.5 ng/dot of double stranded 5-Hydroxymethylcytosine (5-hmC) DNA, 5-Methylcytosine (5-mC) DNA, and unmethylated DNA. The pre-spotted membrane was then blotted with RM231. |
 | Immunocytochemical staining of HeLa cells using rabbit monoclonal anti-5-mC (clone RM231) antibody (red). Actin filaments have been labeled with fluorescein phalloidin (green), and nuclei stained with DAPI (blue). |
 | Direct ELISA of HeLa cell genomic DNA using anti-5-mC antibody (RM231). The plate was directly coated with different concentrations of genomic DNA isolated from HeLa cells. 1 µg/mL or 3 µg/mL of RM231 was used as the primary antibody, and a HRP conjugated anti-rabbit IgG as the secondary antibody. |
 | MeDIP was performed using anti-5-mC antibody (RM231) at a 2:1 DNA: Ab ratio. 1 ng of unmethylated, 5-Methylcytosine (5-mC) or 5-Hydroxymethylcytosine (5-hmC) DNA standard (897 bp) was spiked in 1 µg of genomic DNA isolated from HeLa cells as the control.Realtime PCR was then performed to determine the capture of DNA standard as in % of input. |
![Flow Cytometry analysis of 5-mC expression in HEK293 cells using Anti-5-mC rabbit monoclonal antibody<br />[RM231]. The cells were fixed with ice-cold MeOH, permeabilized with 0.5% Triton X-100, denatured with 2N HCl, then stained with<br />RM231 (anti-5-mC, Blue) or with a<br />negative control antibody (RM105, Red).](https://www.revmab.com/wp-content/uploads/2018/04/5-mc-MeOH.jpg) | Flow Cytometry analysis of 5-mC expression in HEK293 cells using Anti-5-mC rabbit monoclonal antibody, Clone RM231. The cells were fixed with ice-cold MeOH, permeabilized with 0.5% Triton X-100, denatured with 2N HCl, then stained with RM231 (anti-5-mC, Blue) or with a negative control antibody, Clone RM105, Red. |
New Products
