anti-7-Ketocholesterol antibody [3F7]

CAT.NO. : ARG22619

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Size:

50 μg

*产品价格可能会有所调整，请以品牌方官网实时更新的价格为准，以确保准确性。

Product Details

概述

产品描述	Mouse Monoclonal antibody [3F7] recognizes 7-Ketocholesterol
反应物种	Other
应用	ICC/IF, WB
特异性	Specific for 7-ketocholesterol modified proteins.
宿主	Mouse
克隆	Monoclonal
克隆号	3F7
同位型	IgG3
靶点名称	7-Ketocholesterol
抗原物种	Others
抗原	Synthetic 7-Ketocholesterol conjugated to KLH.
偶联标记	Un-conjugated

应用说明

应用建议

应用	推荐稀释比
ICC/IF	1:50
WB	1:1000

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Purification with Protein G.
缓冲液	PBS (pH 7.4), 0.9% Sodium azide and 50% Glycerol.
抗菌剂	0.9% Sodium azide
稳定剂	50% Glycerol
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

检测图片 (2)

ARG22619 anti-7-Ketocholesterol antibody [3F7] ICC/IF image
Immunofluorescence: 5% Formaldehyde fixed (5 min) HEK293 cells stained with ARG22619 anti-7-Ketocholesterol antibody [3F7] at 1:50 for 30 - 60 min at RT. Secondary Antibody: Goat anti-Mouse (Alexa Fluor 488) at 1:1500 for 30 - 60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain. (A,C,E,G) Untreated. (B,D,F,H) Cells cultured overnight with 50 µM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Primary antibody. (G,H) Composite.
ARG22619 anti-7-Ketocholesterol antibody [3F7] WB image
Western blot: BSA (0.5 µg), BSA (2.0 µg), 7-ketocholesterol-BSA (0.5 µg), and 7-ketocholesterol-BSA (2.0 µg) stained with ARG22619 anti-7-Ketocholesterol antibody [3F7] at 1:1000 for 2 hours at RT.

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