anti-ACADM antibody

Key features and details

  • 产品描述: Goat Polyclonal antibody recognizes ACADM
  • 反应物种: Hu
  • 应用: IHC-P, WB
  • 特异性: This antibody is expected to recognise both reported isoforms.
  • 宿主: Goat
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: ACADM
  • 抗原物种: Human
  • Brand:
CAT.NO. : ARG63484
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Product Details
概述
产品描述Goat Polyclonal antibody recognizes ACADM
反应物种Hu
应用IHC-P, WB
特异性This antibody is expected to recognise both reported isoforms.
宿主Goat
克隆Polyclonal
同位型IgG
靶点名称ACADM
抗原物种Human
抗原C-RLIVAREHIDKYKN
偶联标记Un-conjugated
別名ACAD1; MCADH; EC 1.3.8.7; Medium-chain specific acyl-CoA dehydrogenase, mitochondrial; MCAD
应用说明
应用建议
应用推荐稀释比
IHC-P3 - 5 µg/ml
WB0.01 - 0.1 µg/ml
应用说明WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
缓冲液Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
抗菌剂0.02% Sodium azide
稳定剂0.5% BSA
浓度0.5 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 34 Human ACADM

Swiss-port # P11310 Human Medium-chain specific acyl-CoA dehydrogenase, mitochondrial

背景介绍This gene encodes the medium-chain specific (C4 to C12 straight chain) acyl-Coenzyme A dehydrogenase. The homotetramer enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Defects in this gene cause medium-chain acyl-CoA dehydrogenase deficiency, a disease characterized by hepatic dysfunction, fasting hypoglycemia, and encephalopathy, which can result in infantile death. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
研究领域Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody
预测分子量47 kDa
翻译后修饰Acetylation at Lys-307 and Lys-311 in proximity of the cofactor-binding sites reduces catalytic activity (By similarity). These sites are deacetylated by SIRT3.
检测图片 (3)
  • ARG63484 anti-ACADM antibody WB image

    Western Blot: Human Heart lysate (RIPA buffer, 35 µg total protein per lane) stained with ARG63484 anti-ACADM antibody at 0.05 µg/ml dilution.

  • ARG63484 anti-ACADM antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human adrenal gland tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63484 anti-ACADM antibody at 3.75 µg/ml dilution followed by AP-staining.

  • ARG63484 anti-ACADM antibody IHC-P image

    Immunohistochemistry: paraffin embedded Human Liver. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63484 anti-ACADM antibody at 3.8 µg/ml dilution followed by AP-staining.

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