anti-ACAT1 antibody [15E5]

CAT.NO. : ARG57118

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Size:

50 μl

*产品价格可能会有所调整，请以品牌方官网实时更新的价格为准，以确保准确性。

Product Details

概述

产品描述	Mouse Monoclonal antibody [15E5] recognizes ACAT1
反应物种	Hu
应用	ICC/IF, WB
宿主	Mouse
克隆	Monoclonal
克隆号	15E5
同位型	IgG1, kappa
靶点名称	ACAT1
抗原物种	Human
抗原	Recombinant fragment around aa. 34-427 of Human ACAT1
偶联标记	Un-conjugated
別名	EC 2.3.1.9; Acetoacetyl-CoA thiolase; MAT; T2; ACAT; THIL; Acetyl-CoA acetyltransferase, mitochondrial

应用说明

应用建议

应用	推荐稀释比
ICC/IF	Assay-dependent
WB	1:1000

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Purification with Protein A.
缓冲液	PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol.
抗菌剂	0.02% Sodium azide
稳定剂	10% Glycerol
浓度	1 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 38 Human ACAT1 Swiss-port # P24752 Human Acetyl-CoA acetyltransferase, mitochondrial
基因名称	ACAT1
全名	acetyl-CoA acetyltransferase 1
背景介绍	This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. [provided by RefSeq, Feb 2009]
生物功能	Plays a major role in ketone body metabolism. [UniProt]
预测分子量	45 kDa
翻译后修饰	Succinylation at Lys-268, adjacent to a coenzyme A binding site. Desuccinylated by SIRT5 (By similarity).

检测图片 (3)

ARG57118 anti-ACAT1 antibody [15E5] ICC/IF image
Immunofluorescence: Hep3B cells line stained with ARG57118 anti-ACAT1 antibody [15E5] at 1:100 (Green).
DAPI (Blue) for nucleus staining.
ARG57118 anti-ACAT1 antibody [15E5] WB image
Western blot: 40 µg of HepG2 and HeLa cell lysates stained with ARG57118 anti-ACAT1 antibody [15E5] at 1:1000.
ARG57118 anti-ACAT1 antibody [15E5] WB image
Western blot: 40 µg of 1) Hep3B, 2) 293T, and 3) A431 cell lysates stained with ARG57118 anti-ACAT1 antibody [15E5] at 1:1000.

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