anti-ACAT1 antibody

Key features and details

  • 产品描述: Rabbit Polyclonal recognizes ACAT1
  • 反应物种: Hu, Ms, Rat
  • 应用: FACS, ICC/IF, IHC-P, WB
  • 宿主: Rabbit
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: ACAT1
  • 抗原物种: Human
  • 抗原: Human ACAT1 recombinant protein (Position: S10-Q404).
  • Brand:
CAT.NO. : ARG44091
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Product Details
概述
产品描述Rabbit Polyclonal recognizes ACAT1
反应物种Hu, Ms, Rat
应用FACS, ICC/IF, IHC-P, WB
宿主Rabbit
克隆Polyclonal
同位型IgG
靶点名称ACAT1
抗原物种Human
抗原Human ACAT1 recombinant protein (Position: S10-Q404).
偶联标记Un-conjugated
別名EC 2.3.1.9; Acetoacetyl-CoA thiolase; MAT; T2; ACAT; THIL; Acetyl-CoA acetyltransferase, mitochondrial
应用说明
应用建议
应用推荐稀释比
FACS1 - 3 µg/10^6 cells
ICC/IF5 µg/ml
IHC-P2 - 5 µg/ml
WB0.25 - 0.5 µg/ml
应用说明The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Affinity purification with immunogen.
缓冲液0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose.
抗菌剂0.05% Sodium azide
稳定剂4% Trehalose
浓度0.5 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 110446 Mouse ACAT1

GeneID: 25014 Rat ACAT1

GeneID: 38 Human ACAT1

基因名称ACAT1
全名acetyl-CoA acetyltransferase 1
背景介绍This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. [provided by RefSeq, Feb 2009]
生物功能Plays a major role in ketone body metabolism. [UniProt]
预测分子量45 kDa
翻译后修饰Succinylation at Lys-268, adjacent to a coenzyme A binding site. Desuccinylated by SIRT5 (By similarity).
检测图片 (8)
  • ARG44091 anti-ACAT1 antibody IHC-P image

    Immunohistochemistry: Human liver cancer stained with ARG44091 anti-ACAT1 antibody at 2 μg/ml dilution.

  • ARG44091 anti-ACAT1 antibody ICC/IF image

    Immunofluorescence: HeLa stained with ARG44091 anti-ACAT1 antibody at 5 μg/ml dilution.

  • ARG44091 anti-ACAT1 antibody WB image

    Western blot: HepG2,PC-3,A549 and MCF-7 stained with ARG44091 anti-ACAT1 antibody at 0.5 μg/mL dilution.

  • ARG44091 anti-ACAT1 antibody FACS image

    Flow Cytometry: MCF-7 stained with ARG44091 anti-ACAT1 antibody at 1 µg/10^6 cells dilution.

  • ARG44091 anti-ACAT1 antibody WB image

    Western blot: Rat heart stained with ARG44091 anti-ACAT1 antibody at 0.5 μg/mL dilution.

  • ARG44091 anti-ACAT1 antibody WB image

    Western blot: Mouse heart stained with ARG44091 anti-ACAT1 antibody at 0.5 μg/mL dilution.

  • ARG44091 anti-ACAT1 antibody IHC-P image

    Immunohistochemistry: Human placenta stained with ARG44091 anti-ACAT1 antibody at 2 μg/ml dilution.

  • ARG44091 anti-ACAT1 antibody IHC-P image

    Immunohistochemistry: Human non-small cell lung stained with ARG44091 anti-ACAT1 antibody at 2 μg/ml dilution.

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