Anti-Acetyl-Histone H3 (Lys14) Rabbit Monoclonal Antibody [Clone RM130]; H3K14ac; Histone H3 (K14 acetylation); 31-1032-00; 31-1032-00-S
![Anti-Acetyl-Histone H3 (Lys14) Rabbit Monoclonal Antibody [Clone RM130]; H3K14ac; Histone H3 (K14 acetylation); 31-1032-00; 31-1032-00-S](/upload/image/products/WB.png)
![Anti-Acetyl-Histone H3 (Lys14) Rabbit Monoclonal Antibody [Clone RM130]; H3K14ac; Histone H3 (K14 acetylation); 31-1032-00; 31-1032-00-S](/upload/image/products/rm130_2.png)
![Anti-Acetyl-Histone H3 (Lys14) Rabbit Monoclonal Antibody [Clone RM130]; H3K14ac; Histone H3 (K14 acetylation); 31-1032-00; 31-1032-00-S](/upload/image/products/rm130_1.png)
![Anti-Acetyl-Histone H3 (Lys14) Rabbit Monoclonal Antibody [Clone RM130]; H3K14ac; Histone H3 (K14 acetylation); 31-1032-00; 31-1032-00-S](/upload/image/products/Fig4_e.png)
![Anti-Acetyl-Histone H3 (Lys14) Rabbit Monoclonal Antibody [Clone RM130]; H3K14ac; Histone H3 (K14 acetylation); 31-1032-00; 31-1032-00-S](/upload/image/products/Fig-5-RM130-ICC.png)
Key features and details
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Product Details
| Product Name | Anti-Acetyl-Histone H3 (Lys14) Rabbit Monoclonal Antibody, Clone RM130 |
| Product Description | Rabbit monoclonal to Acetylated Histone H3 Lysine 14 (K14ac), H3K14ac, Histone H3 (K14 acetylation) |
| Catalog No. | 31-1032-00 |
| Clone Name | RM130 |
| Product Data Sheet | Anti-Acetyl-Histone H3 Lys14 Rabbit Monoclonal Antibody Data Sheet RM130 |
| Specificity | RM130 reacts to Histone H3 acetylated at Lysine 14 (K14ac), and is not affected by the modification of neighboring amino acids. No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac),or lysine 79 (K79ac) in histone H3. |
| Immunogen | An acetyl-peptide corresponding to Acetyl-Histone H3 (Lys14) |
| Application | Western Blot (WB) Immunocytochemistry (ICC) Chromatin IP (ChIP) ELISA Multiplex |
| Species Reactivity | Human Vertebrates |
| Conjugate | None |
| Intended Use | Research Use Only |
| . | |
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Size | 100 µg |
| Concentration | 1.0 mg/mL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | Western Blot: 0.5 µg/mL - 2 µg/mL; ICC: 0.5 µg/mL - 2 µg/mL; ChIP: 2 µg/mL- 10 µg/mL; ELISA: 0.2 µg/mL - 1 µg/mL; Multiplex: 0.1 µg/mL – 0.5 µg/mL. |
| Keywords | |
| . | |
 | RM130 specifically reacts to Histone H3 acetylated at Lysine 14 (K14ac). No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), or lysine 79 (K79ac) in histone H3. |
 | The binding specificity of RM130 to Histone H3 acetylated at Lysine 14 (K14ac) is not affected by the modification of neighboring amino acids. |
 | Western Blot of acid extracts from HeLa cells untreated (-) or treated with sodium butyrate (+) or, using RM130 at 0.5 µg/mL. |
 | ChIP performed on HeLa cells using H3K14ac antibody (RM130, 5 µg). Real-time PCR was performed using primers specific to the gene indicated. |
 | Immunocytochemistry of HeLa cells treated with sodium butyrate, using Acetyl-Histone H3 (Lys14) Rabbit mAb RM130 (red). Actin filaments have been labeled with fluorescein phalloidin (green). |
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