Anti-Acetyl-Histone H3 (Lys23) Rabbit Monoclonal Antibody [Clone RM169]; H3K23ac; Histone H3 (K23 acetylation); 31-1087-00; 31-1087-00-S
![Anti-Acetyl-Histone H3 (Lys23) Rabbit Monoclonal Antibody [Clone RM169]; H3K23ac; Histone H3 (K23 acetylation); 31-1087-00; 31-1087-00-S](/upload/image/products/Fig-3_ICC_H3K23ac.png)
![Anti-Acetyl-Histone H3 (Lys23) Rabbit Monoclonal Antibody [Clone RM169]; H3K23ac; Histone H3 (K23 acetylation); 31-1087-00; 31-1087-00-S](/upload/image/products/Fig-1_RM169_e.png)
![Anti-Acetyl-Histone H3 (Lys23) Rabbit Monoclonal Antibody [Clone RM169]; H3K23ac; Histone H3 (K23 acetylation); 31-1087-00; 31-1087-00-S](/upload/image/products/Fig-2_RM169_e.png)
Key features and details
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Product Details
| Product Name | Anti-Acetyl-Histone H3 (Lys23) Rabbit Monoclonal Antibody, Clone RM169 |
| Product Description | Rabbit monoclonal to Acetylated Histone H3 Lysine 23 (K23ac), H3K23ac, Histone H3 (K23 acetylation) |
| Catalog No. | 31-1087-00 |
| Clone Name | RM169 |
| Product Data Sheet | Anti-Acetyl-Histone H3 Lys23 Rabbit Monoclonal Antibody Data Sheet RM169 |
| Specificity | RM169 reacts to Histone H3 acetylated at Lysine 23 (K23ac). No cross reactivity with other acetylated Lysines in histone H3. |
| Immunogen | An acetyl-peptide corresponding to Acetyl-Histone H3 (Lys23). |
| Application | Western Blot (WB) ELISA Multiplex Immunocytochemistry (ICC) |
| Species Reactivity | Human Vertebrates |
| Conjugate | None |
| Intended Use | Research Use Only |
| . | |
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Size | 100 µg |
| Concentration | 1.0 mg/mL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | Western Blot: 0.5 µg/mL - 2 µg/mL; ELISA: 0.5 µg/mL - 1 µg/mL; Multiplex: 0.2 µg/mL – 1 µg/mL; Immunocytochemistry: 1 µg/mL - 2 µg/mL. |
| Keywords | |
| References for Acetyl-Histone H3 (Lys23) antibody [RM169] | Cheung P et al. Single-Cell Chromatin Modification Profiling Reveals Increased Epigenetic Variations with Aging. Cell. (2018) 10.1016/j.cell.2018.03.079. Mass Cytometry; Human.Read more (PubMed: 29706550) |
 | Western Blot of acid extracts of HeLa cells non-treated (1) or treated with sodium butyrate (2), and recombinant histone H3.3 (3), using RM169 at 1 µg/mL, showed a band of histone H3 acetylated at Lysine 23 in treated HeLa. |
 | RM169 specifically reacts to Histone H3 acetylated at Lysine 23 (K23ac). No cross reactivity with unmodified Lysine 23 (K23 ctrl), acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14 (K14ac), Lysine 18 (K18ac), Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56 (K56ac), lysine 79 (K79ac), or Lysine 122 (K122) in histone H3. |
 | Immunocytochemical staining of HeLa cells treated with sodium butyrate, using anti-Acetyl-Histone H3 (Lys23) (Clone RM169) (red). Actin filaments have been labeled with fluorescein phalloidin (green), and nuclei stained with DAPI (blue). |
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