Anti-Acetyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody [Clone RM149]; H3K4ac; Histone H3 (K4 acetylation); ChIP Validated; 31-1063-00; 31-1063-00-S
![Anti-Acetyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody [Clone RM149]; H3K4ac; Histone H3 (K4 acetylation); ChIP Validated; 31-1063-00; 31-1063-00-S](/upload/image/products/Fig2_RM149_WB.png)
![Anti-Acetyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody [Clone RM149]; H3K4ac; Histone H3 (K4 acetylation); ChIP Validated; 31-1063-00; 31-1063-00-S](/upload/image/products/Fig1_RM149_E.png)
![Anti-Acetyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody [Clone RM149]; H3K4ac; Histone H3 (K4 acetylation); ChIP Validated; 31-1063-00; 31-1063-00-S](/upload/image/products/Fig3a_RM149_ICC.png)
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CAT.NO. : 31-1063-00
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Product Details
Product Details
| Product Name | Anti-Acetyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody, Clone RM149 |
| Product Description | Rabbit monoclonal to Acetylated Histone H3 Lysine 4 (K4ac), H3K4ac, Histone H3 (K4 acetylation) |
| Catalog No. | 31-1063-00 |
| Clone Name | RM149 |
| Product Data Sheet | Anti-Acetyl-Histone H3 Lys4 Rabbit Monoclonal Antibody Data Sheet RM149 |
| Specificity | RM149 reacts to Histone H3 acetylated at Lysine 4 (K4ac). No cross reactivity with non-modified Lysine 4 or other acetylated Lysines in histone H3. |
| Immunogen | An acetyl-peptide corresponding to Acetyl-Histone H3 (Lys4). |
| Application | Chromatin IP (ChIP) Western Blot (WB) Immunocytochemistry (ICC) ELISA Multiplex |
| Species Reactivity | Human Vertebrates |
| Conjugate | None |
| Intended Use | Research Use Only |
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Properties
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Size | 100 µg |
| Concentration | 1.0 mg/mL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | ChIP: 1 µg - 5 µg; Western Blot: 0.25 µg/mL - 1 µg/mL; ICC:0.5 µg/mL - 2 µg/mL; ELISA: 0.2 µg/mL - 1 µg/mL; Multiplex: 0.05 µg/mL – 0.5 µg/mL. |
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| References | Mondal B et al The histone deacetylase complex MiDAC regulates a neurodevelopmental gene expression program to control neurite outgrowth. Elife. (2020), 10.7554/eLife.57519. WB; Human. Read more (PubMed: 32297854) |
Product Images
 | RM149 specifically reacts to Histone H3 acetylated at Lysine 4 (K4ac). No cross reactivity with non-modified Lysine 4, acetylated Lysine 9 (K9ac), Lysine14 (K14ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56 (K56ac), lysine 79 (K79ac), or Lysine 122 (K122) in histone H3. |
 | Western Blot of acid extracts from HeLa cells untreated (-) or treated with sodium butyrate (+), using RM149 at 0.5 µg/mL, showed a band of histone H3 acetylated at Lysine 4 in treated HeLa. |
 | Immunocytochemistry of HeLa cells treated with sodium butyrate, using Acetyl-Histone H3 (Lys4) Rabbit mAb RM149 (red). Actin filaments have been labeled with fluorescein phalloidin (green). |
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