Anti-Acetyl-Histone H4 (Lys20) Rabbit Monoclonal Antibody [Clone RM205]; H4K20ac; Histone H4 (K20 acetylation); ChIP Validated; 31-1084-00; 31-1084-00-S
![Anti-Acetyl-Histone H4 (Lys20) Rabbit Monoclonal Antibody [Clone RM205]; H4K20ac; Histone H4 (K20 acetylation); ChIP Validated; 31-1084-00; 31-1084-00-S](/upload/image/products/Fig-3_ICC_H4K20ac.png)
![Anti-Acetyl-Histone H4 (Lys20) Rabbit Monoclonal Antibody [Clone RM205]; H4K20ac; Histone H4 (K20 acetylation); ChIP Validated; 31-1084-00; 31-1084-00-S](/upload/image/products/Fig-1_RM205_e.png)
![Anti-Acetyl-Histone H4 (Lys20) Rabbit Monoclonal Antibody [Clone RM205]; H4K20ac; Histone H4 (K20 acetylation); ChIP Validated; 31-1084-00; 31-1084-00-S](/upload/image/products/Fig-2_RM205_e.png)
Key features and details
- Uniprot:
- Gene ID:
- Host Species:
- Species Reactivity:
- Concentration:
-
Brand:
CAT.NO. : 31-1084-00
US＄ Please choose
US＄ Please choose
*产品价格可能会有所调整，请以品牌方官网实时更新的价格为准，以确保准确性。
Product Details
Product Details
| Product Name | Anti-Acetyl-Histone H4 (Lys20) Rabbit Monoclonal Antibody, Clone RM205 |
| Product Description | Rabbit monoclonal to Acetylated Histone H4 Lysine 20 (K20ac), H4K20ac, Histone H4 (K20 acetylation) |
| Catalog No. | 31-1084-00 |
| Clone Name | RM205 |
| Product Data Sheet | Anti-Acetyl-Histone H4 Lys20 Rabbit Monoclonal Antibody Data Sheet RM205 |
| Specificity | RM205 reacts to Histone H4 acetylated at Lysine 20 (K20ac). No cross reactivity with other acetylated Lysines in Histone H4. |
| Immunogen | An acetyl-peptide corresponding to Acetyl-Histone H4 (Lys20). |
| Application | Chromatin IP (ChIP) Western Blot (WB) ELISA Multiplex Immunocytochemistry (ICC) |
| Species Reactivity | Human Vertebrates |
| Conjugate | None |
| Intended Use | Research Use Only |
| . | |
Properties
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Size | 100 µg |
| Concentration | 1.0 mg/mL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | ChIP: 1 µg - 5 µg; Western Blot: 1 µg/mL - 2 µg/mL; ELISA: 0.2 µg/mL - 1 µg/mL; Multiplex: 0.1 µg/mL – 1 µg/mL; Immunocytochemistry: 1 µg/mL - 2 µg/mL. |
| Keywords | |
| . | |
| References | Mondal B et al The histone deacetylase complex MiDAC regulates a neurodevelopmental gene expression program to control neurite outgrowth. Elife. (2020), 10.7554/eLife.57519. WB; Human. Read more (PubMed: 32297854) |
Product Images
 | Western Blot of (1) acid extracts of HeLa cells; (2) recombinant Histone H4. Using RM205 at 1 µg/mL, showed a band of Histone H4 acetylated at Lysine 20 in HeLa cells. |
 | RM205 specifically reacts to Histone H4 acetylated at Lysine 20 (K20ac). No cross reactivity with unmodified Lysine 20 (K20 ctrl), acetylated Lysine 5 (K5ac), Lysine 8 (K8ac), Lysine 12 (K12ac), Lysine 16(K20ac), Lysine 31 (K31ac), or Lysine 91 (K91) in Histone H4. |
 | Immunocytochemical staining of HeLa cells treated with sodium butyrate, using anti-Acetyl-Histone H4 (Lys20) Rabbit Monoclonal Antibody, Clone RM205 (red). Actin filaments have been labeled with fluorescein phalloidin (green). |
New Products
