Anti-Acetyl-Histone H4 (Lys5) Rabbit Monoclonal Antibody [Clone RM199]; H4K5ac; Histone H4 (K5 acetylation); 31-1081-00; 31-1081-00-S
![Anti-Acetyl-Histone H4 (Lys5) Rabbit Monoclonal Antibody [Clone RM199]; H4K5ac; Histone H4 (K5 acetylation); 31-1081-00; 31-1081-00-S](/upload/image/products/Fig-3_ICC_H4K8ac-e1472082705387.png)
![Anti-Acetyl-Histone H4 (Lys5) Rabbit Monoclonal Antibody [Clone RM199]; H4K5ac; Histone H4 (K5 acetylation); 31-1081-00; 31-1081-00-S](/upload/image/products/Fig-1_RM199_e.png)
![Anti-Acetyl-Histone H4 (Lys5) Rabbit Monoclonal Antibody [Clone RM199]; H4K5ac; Histone H4 (K5 acetylation); 31-1081-00; 31-1081-00-S](/upload/image/products/Fig-2_RM199_e.png)
Key features and details
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CAT.NO. : 31-1081-00
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Product Details
Product Details
| Product Name | Anti-Acetyl-Histone H4 (Lys5) Rabbit Monoclonal Antibody, Clone RM199 |
| Product Description | Rabbit monoclonal to Acetylated Histone H4 Lysine 5 (K5ac), H4K5ac, Histone H4 (K5 acetylation) |
| Catalog No. | 31-1081-00 |
| Clone Name | RM199 |
| Product Data Sheet | Anti-Acetyl-Histone H4 Lys5 Rabbit Monoclonal Antibody Data Sheet RM199 |
| Specificity | RM199 reacts to Histone H4 acetylated at Lysine 5 (K5ac). No cross reactivity with other acetylated Lysines in Histone H4. |
| Immunogen | An acetyl-peptide corresponding to Acetyl-Histone H4 (Lys5). |
| Application | Western Blot (WB) Immunocytochemistry (ICC) ELISA Multiplex |
| Species Reactivity | Human Vertebrates |
| Conjugate | None |
| Intended Use | Research Use Only |
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Properties
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Size | 100 µg |
| Concentration | 1.0 mg/mL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | Western Blot: 0.2 µg/mL - 1 µg/mL; Immunocytochemistry: 0.5 µg/mL - 2 µg/mL.; ELISA: 0.2 µg/mL - 1 µg/mL; Multiplex: 0.05 µg/mL – 0.2 µg/mL. |
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Product Images
 | RM199 specifically reacts to Histone H4 acetylated at Lysine 5 (K5ac). No cross reactivity with unmodified Lysine 5 (K5 ctrl), acetylated Lysine 8 (K8ac), Lysine 12 (K12ac), Lysine 16 (K16ac), Lysine 20 (K20ac), Lysine 31 (K31ac), or Lysine 91 (K91) in Histone H4. |
 | Western Blot of (1) acid extracts of HeLa cells; (2) recombinant Histone H4. Using RM199 at 0.2 µg/mL, showed a band of Histone H4 acetylated at Lysine 5 in HeLa cells. |
 | Immunocytochemical staining of HeLa cells treated with sodium butyrate, using anti-Acetyl-Histone H4 (Lys5) Rabbit Monoclonal Antibody, Clone RM199 (red). Actin filaments have been labeled with fluorescein phalloidin (green). |
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