anti-Acetylated Lysine antibody [7F8]
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![anti-Acetylated Lysine antibody [7F8]](/upload/image/products/ARG20521_IHC_Mouse_backskin_1.jpg)
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Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 宿主:
- 克隆:
- 克隆号:
- 同位型:
- 靶点名称:
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Brand:
CAT.NO. : ARG20521
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Product Details
概述
| 产品描述 | Mouse Monoclonal antibody [7F8] recognizes Acetylated Lysine |
|---|---|
| 反应物种 | Other |
| 应用 | ELISA, ICC/IF, IHC, IP, WB |
| 特异性 | Detects proteins containing acetylated lysine residues. Does not detect non-acetylated lysine residues. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 7F8 |
| 同位型 | IgG1 |
| 靶点名称 | Acetylated Lysine |
| 抗原 | Acetylated KLH |
| 偶联标记 | Un-conjugated |
应用说明
| 交叉反应性说明 | Species Independent. Bovine albumin and Avian histones have been tested. | ||||||||||||
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| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS (pH 7.4), 0.09% Sodium azide and 50% Glycerol |
| 抗菌剂 | 0.09% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 背景介绍 | Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common posttranscriptional modification of Lysine involves acetylation (1). The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2). Protein posttranslational reversible lysine N ε - acetylation and deacetylation have been recognized as an emerging intracellular signaling mechanism that plays critical roles in regulating gene transcription, cell-cycle progression, apoptosis, DNA repair, and cytoskeletal organization (3). The regulation of protein acetylation status is impaired in the pathologies of cancer and polyglutamine diseases (4), and HDACs have become promising targets for anticancer drugs currently in development (5). 1. Yang XJ. (2005). Oncogene. 24:1653-1662. 2. Hassig, C.A. and Schreiber, S.L. (1997). Curr. Opin. Chem. Biol. 1(3): 300-308. 3. Yang XJ. (2004). Bioessays 26:1076-1087. 4. Hughes, R.E. (2002). Curr. Biol. 12: R141-R143. 5. Vigushin, D.M. and Coombes, R.C. (2004). Curr. Cancer Drug Targets 4: 205-218. |
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| 研究领域 | Gene Regulation antibody |
检测图片 (3)
ARG20521 anti-Acetylated Lysine antibody [7F8] IHC-P image
Immunohistochemistry: Bouin's fixed and paraffin-embedded Mouse backskin stained with ARG20521 anti-Acetylated Lysine antibody [7F8] at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat anti-Mouse (green) at 1:50 for 1 hour at RT.
ARG20521 anti-Acetylated Lysine antibody [7F8] WB image
Western blot: A549 cell lysate stained with ARG20521 anti-Acetylated Lysine antibody [7F8] at 1:200 dilution.
ARG20521 anti-Acetylated Lysine antibody [7F8] WB image
Western blot: Acetylated lysine in 1) 75 ng of Acetylated BSA, 2) Non-acetylated BSA, and 3) Marker. The blots were stained with ARG20521 anti-Acetylated Lysine antibody [7F8] at 1:1000 dilution.
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