anti-Acetylated Lysine antibody

Key features and details

  • 产品描述: Rabbit Polyclonal antibody recognizes Acetylated Lysine
  • 反应物种: Other
  • 应用: ELISA, ICC/IF, IP, WB
  • 特异性: Detects proteins containing acetylated lysine residues. Does not detect non-acetylated lysine residues.
  • 宿主: Rabbit
  • 克隆: Polyclonal
  • 靶点名称: Acetylated Lysine
  • 抗原: Acetylated KLH
  • 偶联标记: Un-conjugated
  • Brand:
CAT.NO. : ARG20523
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Product Details
概述
产品描述Rabbit Polyclonal antibody recognizes Acetylated Lysine
反应物种Other
应用ELISA, ICC/IF, IP, WB
特异性Detects proteins containing acetylated lysine residues. Does not detect non-acetylated lysine residues.
宿主Rabbit
克隆Polyclonal
靶点名称Acetylated Lysine
抗原Acetylated KLH
偶联标记Un-conjugated
应用说明
交叉反应性说明Species Independent.
应用建议
应用推荐稀释比
ELISAAssay-dependent
ICC/IF1:100
IPAssay-dependent
WB1:250
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Affinity purification with immunogen.
缓冲液PBS, 0.09% Sodium azide and 50% Glycerol
抗菌剂0.09% Sodium azide
稳定剂50% Glycerol
浓度1 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
背景介绍Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common post-transcriptional modification of Lysine involves acetylation (1). The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2). Protein posttranslational reversible lysine Nε-acetylation and deacetylation have been recognized as an emerging intracellular signaling mechanism that plays critical roles in regulating gene transcription, cell-cycle progression, apoptosis, DNA repair, and cytoskeletal organization (3). The regulation of protein acetylation status is impaired in the pathologies of cancer and polyglutamine diseases (4), and HDACs have become promising targets for anti-cancer drugs currently in development (5).

1. Yang XJ. (2005). Oncogene. 24:1653-1662.
2. Hassig, C.A. and Schreiber, S.L. (1997). Curr. Opin. Chem. Biol. 1(3): 300-308.
3. Yang XJ. (2004). Bioessays 26:1076-1087.
4. Hughes, R.E. (2002). Curr. Biol. 12: R141-R143.
5. Vigushin, D.M. and Coombes, R.C. (2004). Curr. Cancer Drug Targets 4: 205-218.
6. Chan, H.M. et al. (2001). Nat. Cell Biol. 3: 667- . 674.
7. Martinez-Balbas, M.A. et al. (2000). EMBO J. 19: 662-671.
研究领域Gene Regulation antibody
检测图片 (4)
  • ARG20523 anti-Acetylated Lysine antibody ICC/IF image

    Immunofluorescence: Heat Shocked (42°C for 1 hour) HeLa cells. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: ARG20523 anti-Acetylated Lysine antibody at 1:100 for 12 hours at 4°C. Secondary Antibody: R-PE Goat anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Magnification: 100x. Left: DAPI (blue) nuclear stain. Middle: ARG20523 anti-Acetylated Lysine antibody. Right: Composite.

  • ARG20523 anti-Acetylated Lysine antibody WB image

    Western blot: acetylated histone from TSA-treated mouse spleen cells, stained with ARG20523 anti-Acetylated Lysine antibody.

  • ARG20523 anti-Acetylated Lysine antibody ICC/IF image

    Immunofluorescence: Heat Shocked (42°C for 1 hour) HeLa cells. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: ARG20523 anti-Acetylated Lysine antibody at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Magnification: 20x. Left: DAPI (blue) nuclear stain. Middle: ARG20523 anti-Acetylated Lysine antibody. Right: Composite.

  • ARG20523 anti-Acetylated Lysine antibody WB image

    Western blot: acetylated lysine in BSA (Left) and Acetylated BSA (Right) stained with ARG20523 anti-Acetylated Lysine antibody at 1:1000 dilution.

参考文献

Sirtuin 6 Deacetylates Apoptosis-Associated Speck-Like Protein (ASC) to Inhibit Endothelial Cell Pyroptosis in Atherosclerosis

WB / Human

Jian Huang et al.
Int Heart J.,  (2024)

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Interfering TRIB3 protects the blood brain barrier through PI3K/Akt pathway to alleviate cerebral ischemia-reperfusion injury in diabetes mellitus mice

IP / Mouse

Heng-Jun Zhou et al.
Chem Biol Interact.,  (2024)

publication_link

 

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