Anti-Acetylated-Lysine Rabbit Monoclonal Antibody [Clone RM101]; 32-1012-00
![Anti-Acetylated-Lysine Rabbit Monoclonal Antibody [Clone RM101]; 32-1012-00](/upload/image/products/Fig3_ICC_RM101_e.png)
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![Anti-Acetylated-Lysine Rabbit Monoclonal Antibody [Clone RM101]; 32-1012-00](/upload/image/products/anti-ace-3.jpg)
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CAT.NO. : 32-1012-00
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Product Details
Product Details
| Product Name | Anti-Acetylated-Lysine Rabbit Monoclonal Antibody, Clone RM101 |
| Description | Rabbit monoclonal to Acetylated-Lysine |
| Catalog No. | 32-1012-00 |
| Clone Name | RM101 |
| Product Data Sheet | Anti-Acetylated Lysine Rabbit Monoclonal Antibody Data Sheet RM101 |
| Specificity | RM101 reacts to lysine-acetylated proteins. No cross reactivity with nonacetylated lysine, and lysines with other modifications. |
| Immunogen | Acetyllysine-BSA |
| Application | Western Blot (WB) Immunoprecipitation (IP) Immunohistochemistry (IHC) Flow Cytometry (FC) Immunocytochemistry (ICC) ELISA |
| Species Reactivity | All Species |
| Conjugate | None |
| Intended Use | Research Use Only |
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Properties
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Item Size | 100 µg |
| Concentration | 1.0 mg/mL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | Western Blot (WB): 1:500 -1:2000 dilution; Immunoprecipitation (IP): 1:100-1:500 dilution; Chromatin IP (ChIP): 1:100-1:500 dilution; Immunocytochemistry (ICC): 1:100-1:500 dilution; Immunohistochemistry (IHC): 1:100-1:500 dilution. |
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Product Images
 | Immunocytochemical staining of HeLa cells using Anti-Acetylated Lysine antibody, Clone RM101, (red).Actin filaments was labeled with fluorescein phalloidin (green), and nucleus stained with DAPI (blue). |
 | Western blot of HeLa cells nontreated or treated with Trichostatin A (TSA), using RevMAb Clone RM101 at 1/2000 dilution. |
 | Immunoprecipitation of A431 cell lysates, using RevMAb Clone RM101 at 1/500 dilution, was blotted with anti-PTEN mouse monoclonal antibody. |
 | RevMAb rabbit monoclonal antibody, Clone RM101, recognizes acetylated lysine in peptides with different sequences. It does not react with non-acetylated lysine. |
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