anti-ACOT7 antibody [1D5]

Key features and details

  • 产品描述: Mouse Monoclonal antibody [1D5] recognizes ACOT7
  • 反应物种: Hu
  • 应用: FACS, ICC/IF, WB
  • 宿主: Mouse
  • 克隆: Monoclonal
  • 克隆号: 1D5
  • 同位型: IgG2a, kappa
  • 靶点名称: ACOT7
  • 抗原物种: Human
  • Brand:
CAT.NO. : ARG57015
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Product Details
概述
产品描述Mouse Monoclonal antibody [1D5] recognizes ACOT7
反应物种Hu
应用FACS, ICC/IF, WB
宿主Mouse
克隆Monoclonal
克隆号1D5
同位型IgG2a, kappa
靶点名称ACOT7
抗原物种Human
抗原Recombinant fragment around aa. 1-370 of Human ACOT7.
偶联标记Un-conjugated
別名EC 3.1.2.2; CTE-IIa; hBACH; LACH; Cytosolic acyl coenzyme A thioester hydrolase; CTE-II; ACH1; BACH; Long chain acyl-CoA thioester hydrolase; LACH1; Acyl-CoA thioesterase 7; ACT; Brain acyl-CoA hydrolase
应用说明
应用建议
应用推荐稀释比
FACSAssay-dependent
ICC/IFAssay-dependent
WB1:500 - 1:2000
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Purification with Protein A.
缓冲液PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol.
抗菌剂0.02% Sodium azide
稳定剂10% Glycerol
浓度1 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 11332 Human ACOT7

Swiss-port # O00154 Human Cytosolic acyl coenzyme A thioester hydrolase

基因名称ACOT7
全名acyl-CoA thioesterase 7
背景介绍This gene encodes a member of the acyl coenzyme family. The encoded protein hydrolyzes the CoA thioester of palmitoyl-CoA and other long-chain fatty acids. Decreased expression of this gene may be associated with mesial temporal lobe epilepsy. Alternatively spliced transcript variants encoding distinct isoforms with different subcellular locations have been characterized. [provided by RefSeq, Jul 2008]
生物功能Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. May play an important physiological function in brain. May play a regulatory role by modulating the cellular levels of fatty acyl-CoA ligands for certain transcription factors as well as the substrates for fatty acid metabolizing enzymes, contributing to lipid homeostasis. Has broad specificity, active towards fatty acyl-CoAs with chain-lengths of C8-C18. Has a maximal activity toward palmitoyl-CoA. [UniProt]
预测分子量42 kDa
检测图片 (3)
  • ARG57015 anti-ACOT7 antibody [1D5] ICC/IF image

    Immunoflorescense: U87MG cell line stained with ARG57015 anti-ACOT7 antibody [1D5] at 1:100 (Green).

    DAPI (Blue) for nucleus staining.

  • ARG57015 anti-ACOT7 antibody [1D5] FACS image

    Flow Cytometry: 293T cell line stained with ARG57015 anti-ACOT7 antibody [1D5] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was Mouse IgG (black line).

  • ARG57015 anti-ACOT7 antibody [1D5] WB image

    Western blot: 30 µg of 293T cell lysate stained with ARG57015 anti-ACOT7 antibody [1D5] at 1:1000.

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