Anti-β-Actin Rabbit Monoclonal Antibody [Clone RM112]; 31-1013-00
![Anti-β-Actin Rabbit Monoclonal Antibody [Clone RM112]; 31-1013-00](/upload/image/products/ICC_actin_HeLa.png)
![Anti-β-Actin Rabbit Monoclonal Antibody [Clone RM112]; 31-1013-00](/upload/image/products/RM112_WB_A431-e1649288208449.jpg)
Key features and details
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CAT.NO. : 31-1013-00
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Product Details
Product Details
| Product Name | Anti-β-Actin Rabbit Monoclonal Antibody, Clone RM112 |
| Description | Rabbit monoclonal to β-Actin (beta-actin) |
| Catalog No. | 31-1013-00 |
| Clone Name | RM112 |
| Product Data Sheet | Anti-Beta Actin Rabbit Monoclonal Antibody Data Sheet RM112 |
| Specificity | RM112 reacts toβ-Actin (Actin, cytoplasmic 1). No cross reactivity with other isoforms of Actin. |
| Immunogen | A peptide corresponding to the N-terminus of β-actin |
| Application | Western Blot (WB) Immunoprecipitation (IP) Immunohistochemistry (IHC) Flow Cytometry (FC) Immunocytochemistry (ICC) ELISA |
| Species Reactivity | All Species of Animals |
| Conjugate | None |
| Intended Use | Research Use Only |
| . | |
Properties
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Item Size | 100 µL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | Western Blot (WB): 1:1000 dilution; Immunoprecipitation (IP): 1:200 dilution; Chromatin IP (ChIP): 1:200 dilution; Immunocytochemistry (ICC): 1:200 dilution; Immunohistochemistry (IHC): 1:200-1:1000 dilution. |
| Keywords | |
| References for β-Actin Rabbit Monoclonal Antibody [RM112] | Jevtić P et al. The nucleoporin ELYS regulates nuclear size by controlling NPC number and nuclear import capacity. EMBO Rep. (2019), 10.15252/embr.201847283. WB; Human. Read more (PubMed: 31085625) Wang G et al. Structural plasticity of actin-spectrin membrane skeleton and functional role of actin and spectrin in axon degeneration. Elife. (2019), 10.7554/eLife.38730. WB; Human. Read more (PubMed: 31042147) Mukherjee RN et al. Reticulon 4a promotes exocytosis in mammalian cells. Elife. (2019), 10.1091/mbc.E19-03-0159. WB; Human. Read more (PubMed: 31318320) |
Product Images
 | Immunohistochemical staining of formalin fixed and paraffin embedded human tonsil tissue using Anti-beta actin Rabbit Monoclonal Antibody, Clone RM112, at a 1:1000 dilution. |
 | Immunocytochemical staining of HeLa cells, using anti-β-Actin RevMAb Clone RM112 at 1/200 dilution (red).Nuclei have been labeled with DAPI (blue). |
 | Western blot of A431 cells, using anti-β-Actin RevMAb Clone RM112 at a 1/1000 dilution, showed a band of β-Actin at the predicted MW (42 kDa). |
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