anti-ACY1 / Aminoacylase 1 antibody [1E2]

CAT.NO. : ARG57036

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Size:

50 μl

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Product Details

概述

产品描述	Mouse Monoclonal antibody [1E2] recognizes ACY1 / Aminoacylase 1
反应物种	Hu
应用	FACS, WB
宿主	Mouse
克隆	Monoclonal
克隆号	1E2
同位型	IgG2b, kappa
靶点名称	ACY1 / Aminoacylase 1
抗原物种	Human
抗原	Recombinant fragment around aa. 1-408 of Human Aminoacylase 1.
偶联标记	Un-conjugated
別名	ACY-1; N-acyl-L-amino-acid amidohydrolase; ACY1D; EC 3.5.1.14; HEL-S-5; Aminoacylase-1

应用说明

应用建议

应用	推荐稀释比
FACS	Assay-dependent
WB	1:250 - 1:500

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Purification with Protein A.
缓冲液	PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol.
抗菌剂	0.02% Sodium azide
稳定剂	10% Glycerol
浓度	1 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 95 Human ACY1 Swiss-port # Q03154 Human Aminoacylase-1
基因名称	ACY1
全名	aminoacylase 1
背景介绍	This gene encodes a cytosolic, homodimeric, zinc-binding enzyme that catalyzes the hydrolysis of acylated L-amino acids to L-amino acids and an acyl group, and has been postulated to function in the catabolism and salvage of acylated amino acids. This gene is located on chromosome 3p21.1, a region reduced to homozygosity in small-cell lung cancer (SCLC), and its expression has been reported to be reduced or undetectable in SCLC cell lines and tumors. The amino acid sequence of human aminoacylase-1 is highly homologous to the porcine counterpart, and this enzyme is the first member of a new family of zinc-binding enzymes. Mutations in this gene cause aminoacylase-1 deficiency, a metabolic disorder characterized by central nervous system defects and increased urinary excretion of N-acetylated amino acids. Alternative splicing of this gene results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream ABHD14A (abhydrolase domain containing 14A) gene, as represented in GeneID:100526760. A related pseudogene has been identified on chromosome 18. [provided by RefSeq, Nov 2010]
生物功能	Involved in the hydrolysis of N-acylated or N-acetylated amino acids (except L-aspartate). [UniProt]
预测分子量	46 kDa

检测图片 (3)

ARG57036 anti-ACY1 / Aminoacylase 1 antibody [1E2] FACS image
Flow Cytometry: Hep3B cells stained with ARG57036 anti-ACY1 / Aminoacylase 1 antibody [1E2] at 2-5 µg/10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was Mouse IgG (black line).
ARG57036 anti-ACY1 / Aminoacylase 1 antibody [1E2] WB image
Western blot: 40 µg of Jurkat, HepG2, HeLa, and A549 stained with ARG57036 anti-ACY1 / Aminoacylase 1 antibody [1E2] at 1:500.
ARG57036 anti-ACY1 / Aminoacylase 1 antibody [1E2] WB image
Western blot: 50 ng of Recombinant Human ACY1 stained with ARG57036 anti-ACY1 / Aminoacylase 1 antibody [1E2] at 1:1000.

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