anti-ADRB2 antibody

Key features and details

  • 产品描述: Goat Polyclonal antibody recognizes ADRB2
  • 反应物种: Hu
  • 预测物种: Cow, Dog
  • 应用: WB
  • 宿主: Goat
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: ADRB2
  • 抗原物种: Human
  • Brand:
CAT.NO. : ARG63852
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Product Details
概述
产品描述Goat Polyclonal antibody recognizes ADRB2
反应物种Hu
预测物种Cow, Dog
应用WB
宿主Goat
克隆Polyclonal
同位型IgG
靶点名称ADRB2
抗原物种Human
抗原C-HQGTVPSDNIDSQ
偶联标记Un-conjugated
別名B2AR; BAR; BETA2AR; Beta-2 adrenergic receptor; ADRB2R; ADRBR; Beta-2 adrenoceptor; Beta-2 adrenoreceptor
应用说明
应用建议
应用推荐稀释比
WB0.3 - 1 µg/ml
应用说明WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
缓冲液Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
抗菌剂0.02% Sodium azide
稳定剂0.5% BSA
浓度0.5 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 154 Human ADRB2

Swiss-port # P07550 Human Beta-2 adrenergic receptor

背景介绍This gene encodes beta-2-adrenergic receptor which is a member of the G protein-coupled receptor superfamily. This receptor is directly associated with one of its ultimate effectors, the class C L-type calcium channel Ca(V)1.2. This receptor-channel complex also contains a G protein, an adenylyl cyclase, cAMP-dependent kinase, and the counterbalancing phosphatase, PP2A. The assembly of the signaling complex provides a mechanism that ensures specific and rapid signaling by this G protein-coupled receptor. This gene is intronless. Different polymorphic forms, point mutations, and/or downregulation of this gene are associated with nocturnal asthma, obesity and type 2 diabetes. [provided by RefSeq, Jul 2008]
研究领域Cell Biology and Cellular Response antibody; Signaling Transduction antibody
预测分子量46 kDa
翻译后修饰Palmitoylated; may reduce accessibility of Ser-345 and Ser-346 by anchoring Cys-341 to the plasma membrane. Agonist stimulation promotes depalmitoylation and further allows Ser-345 and Ser-346 phosphorylation.
Phosphorylated by PKA and BARK upon agonist stimulation, which mediates homologous desensitization of the receptor. PKA-mediated phosphorylation seems to facilitate phosphorylation by BARK.
Phosphorylation of Tyr-141 is induced by insulin and leads to supersensitization of the receptor.
Polyubiquitinated. Agonist-induced ubiquitination leads to sort internalized receptors to the lysosomes for degradation (PubMed:19424180, PubMed:20559325). Deubiquitination by USP20 and USP33, leads to ADRB2 recycling and resensitization after prolonged agonist stimulation. USP20 and USP33 are constitutively associated and are dissociated immediately after agonist stimulation. Ubiquitination by the VHL-E3 ligase complex is oxygen-dependent.
Hydroxylation by EGLN3 occurs only under normoxia and increases the interaction with VHL and the subsequent ubiquitination and degradation of ADRB2.
检测图片 (2)
  • ARG63852 anti-ADRB2 antibody WB image

    Western blot: Human liver lysate (35 µg protein in RIPA buffer) stained with ARG63852 anti-ADRB2 antibody at 0.2 µg/ml dilution.

  • ARG63852 anti-ADRB2 antibody WB image

    Western blot: 35 µg of HepG2 cell lysate (in RIPA buffer) stained with ARG63852 anti-ADRB2 antibody at 0.5 µg/ml dilution and incubated at RT for 1 hour.

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