anti-AK3 antibody [SJB3-36]
产品描述 | Mouse Monoclonal antibody [SJB3-36] recognizes AK3 |
---|---|
反应物种 | Hu, Ms |
应用 | FACS, ICC/IF, IHC-P, WB |
宿主 | Mouse |
克隆 | Monoclonal |
克隆号 | SJB3-36 |
同位型 | IgG1, kappa |
靶点名称 | AK3 |
抗原物种 | Human |
抗原 | Recombinant fragment of Human AK3. |
偶联标记 | Un-conjugated |
別名 | EC 2.7.4.10; AKL3L; Adenylate kinase 3; Adenylate kinase 3 alpha-like 1; FIX; AK6; AK3L1; AKL3L1; GTP:AMP phosphotransferase AK3, mitochondrial; AK 3 |
应用建议 |
| ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
形式 | Liquid |
---|---|
纯化 | Purification with Protein G. |
缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
抗菌剂 | 0.02% Sodium azide |
稳定剂 | 10% Glycerol |
浓度 | 1 mg/ml |
存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
数据库连接 | Swiss-port # Q9UIJ7 Human GTP:AMP phosphotransferase AK3, mitochondrial Swiss-port # Q9WTP7 Mouse GTP:AMP phosphotransferase AK3, mitochondrial |
---|---|
基因名称 | AK3 |
全名 | adenylate kinase 3 |
背景介绍 | The protein encoded by this gene is a GTP:ATP phosphotransferase that is found in the mitochondrial matrix. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Dec 2010] |
生物功能 | Involved in maintaining the homeostasis of cellular nucleotides by catalyzing the interconversion of nucleoside phosphates. Has GTP:AMP phosphotransferase and ITP:AMP phosphotransferase activities. [UniProt] |
预测分子量 | 26 kDa |
ARG56921 anti-AK3 antibody [SJB3-36] ICC/IF image
Immunoflorescense: U87MG cell line stained with ARG56921 anti-AK3 antibody [SJB3-36] at 1:100 (Green).
DAPI (Blue) for nucleus staining.
ARG56921 anti-AK3 antibody [SJB3-36] FACS image
Flow Cytometry: Jurkat cell line stained with ARG56921 anti-AK3 antibody [SJB3-36] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was Mouse IgG (black line).
ARG56921 anti-AK3 antibody [SJB3-36] IHC-P image
Immunohistochemistry: Paraffin-embedded sections of Human liver tissue stained with ARG56921 anti-AK3 antibody [SJB3-36] at 1:100 for 2 hours at RT.
ARG56921 anti-AK3 antibody [SJB3-36] WB image
Western blot: 50 ng of 1) Recombinant Human AK1, 2) Recombinant Human AK2, 3) Recombinant Human AK3 stained with ARG56921 anti-AK3 antibody [SJB3-36] at 1:1000. .
ARG56921 anti-AK3 antibody [SJB3-36] WB image
Western blot: 40 µg of 1) Hep3B cell lysate, 2) 293T cell lysate, 3) Mouse kidney tissue lysate stained with ARG56921 anti-AK3 antibody [SJB3-36] at 1:1000.
ARG56921 anti-AK3 antibody [SJB3-36] WB image
Western blot: 40 µg of Mouse liver tissue lysate stained with ARG56921 anti-AK3 antibody [SJB3-36] at 1:1000.
