anti-AKAP9 / AKAP450 / CG-NAP antibody
Key features and details
- 产品描述:
- 反应物种:
- 预测物种:
- 应用:
- 特异性:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
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Brand:
CAT.NO. : ARG63741
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Product Details
概述
| 产品描述 | Goat Polyclonal antibody recognizes AKAP9 / AKAP450 / CG-NAP |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms |
| 应用 | ICC/IF, WB |
| 特异性 | This antibody is expected to recogniseisoforms 2 and 3 (NP_005742.4; NP_671714.1 respectively).The isoforms Yotiao, AKAP350B and AKAP350C are not recognized. |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | AKAP9 / AKAP450 / CG-NAP |
| 抗原物种 | Human |
| 抗原 | C-SGSTTQFHAGMR |
| 偶联标记 | Un-conjugated |
| 別名 | AKAP 120-like protein; Protein kinase A-anchoring protein 9; LQT11; A-kinase anchor protein 450 kDa; CG-NAP; PPP1R45; A-kinase anchor protein 350 kDa; hgAKAP 350; AKAP-9; MU-RMS-40.16A; AKAP350; YOTIAO; Protein yotiao; Protein hyperion; AKAP 350; PRKA9; A-kinase anchor protein 9; AKAP 450; AKAP450; HYPERION; Centrosome- and Golgi-localized PKN-associated protein |
应用说明
| 应用建议 |
| ||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 背景介绍 | The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. Alternate splicing of this gene results in at least two isoforms that localize to the centrosome and the Golgi apparatus, and interact with numerous signaling proteins from multiple signal transduction pathways. These signaling proteins include type II protein kinase A, serine/threonine kinase protein kinase N, protein phosphatase 1, protein phosphatase 2a, protein kinase C-epsilon and phosphodiesterase 4D3. [provided by RefSeq, Aug 2008] |
| 研究领域 | Controls and Markers antibody |
| 预测分子量 | 453 kDa |
检测图片 (2)
ARG63741 anti-AKAP9 / AKAP450 / CG-NAP antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed A431 cells permeabilized with 0.15% Triton. Cells were stained with ARG63741 anti-AKAP9 / AKAP450 / CG-NAP antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
ARG63741 anti-AKAP9 / AKAP450 / CG-NAP antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed U2OS cells permeabilized with 0.15% Triton. Cells were stained with ARG63741 anti-AKAP9 / AKAP450 / CG-NAP antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
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