Anti-Akt (E17K mutant) Rabbit Monoclonal Antibody [Clone RM336], 31-1222-00, 31-1222-02
![Anti-Akt (E17K mutant) Rabbit Monoclonal Antibody [Clone RM336], 31-1222-00, 31-1222-02](/upload/image/products/Fig2_IHC_AktE17K.png)
![Anti-Akt (E17K mutant) Rabbit Monoclonal Antibody [Clone RM336], 31-1222-00, 31-1222-02](/upload/image/products/Fig1_WB_AktE17K.png)
Key features and details
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CAT.NO. : 31-1222-02
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Product Details
Product Details
| Product Name | Anti-Akt (E17K Mutant) Rabbit Monoclonal Antibody, Clone RM336 |
| Product Description | Rabbit monoclonal to Akt (E17K Mutant) |
| Catalog No. | 31-1222-00; 31-1222-02 |
| Clone Name | RM336 |
| Product Data Sheet | Anti-Akt (E17K Mutant) Rabbit Monoclonal Antibody Data Sheet RM336 Biotinylated Anti-Akt (E17K Mutant) Rabbit Monoclonal Antibody Data Sheet RM336 |
| Specificity | RM336 reacts to the Akt E17K mutant. No cross reactivity with wild type Akt. |
| Immunogen | A peptide corresponding to the Akt E17K Mutant |
| Application | Western Blot ELISA Immunohistochemistry |
| Species Reactivity | All |
| Conjugate | None |
| Intended Use | Research Use Only |
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Properties
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Size | 50 µg; Biotinylated 50 µg |
| Concentration | 1.0 mg/mL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | Western Blot: 0.1 µg/mL - 0.5 µg/mL; ELISA: 0.05 µg/mL - 0.5 µg/mL; Immunohistochemistry (IHC): 0.2 µg/mL - 1 µg/mL. |
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Product Images
 | Western Blot analysis of a cell lysate mixture of untransfected 293T and 293T transfected with a DNA construct encoding the Akt E17K mutant, using anti-Akt E17K (Clone RM336) (0.1 µg/mL) or anti-Akt (Clone RM252) rabbit monoclonal antibody. |
 | Immunohistochemical staining of formalin fixed and paraffin embedded 293T cells transfected with a DNA construct encoding the Akt E17K mutation or Histone H3.3, stained with anti-Akt E17K rabbit monoclonal antibody, Clone RM336 at 0.2 µg/mL. |
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