anti-ALDH2 antibody

Key features and details

  • 产品描述: Mouse Monoclonal antibody recognizes ALDH2
  • 反应物种: Hu, Ms, Rat
  • 应用: FACS, ICC/IF, IHC, WB
  • 宿主: Mouse
  • 克隆: Monoclonal
  • 克隆号: 138CT22.3.8
  • 同位型: IgG1, kappa
  • 靶点名称: ALDH2
  • 抗原物种: Human
  • Brand:
CAT.NO. : ARG55209
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Product Details
概述
产品描述Mouse Monoclonal antibody recognizes ALDH2
反应物种Hu, Ms, Rat
应用FACS, ICC/IF, IHC, WB
宿主Mouse
克隆Monoclonal
克隆号138CT22.3.8
同位型IgG1, kappa
靶点名称ALDH2
抗原物种Human
抗原Recombinant protein of Human ALDH2 (Swiss: P05091)
偶联标记Un-conjugated
別名EC 1.2.1.3; ALDH class 2; ALDM; ALDHI; Aldehyde dehydrogenase, mitochondrial; ALDH-E2
应用说明
应用建议
应用推荐稀释比
FACS1:10 - 1:50
ICC/IF1:10 - 1:50
IHC1:50 - 1:100
WB1:1000
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Purification with Protein G.
缓冲液PBS and 0.09% (W/V) Sodium azide
抗菌剂0.09% (W/V) Sodium azide
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 11669 Mouse ALDH2

GeneID: 217 Human ALDH2

GeneID: 29539 Rat ALDH2

基因名称ALDH2
全名aldehyde dehydrogenase 2 family (mitochondrial)
背景介绍This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of aldehyde dehydrogenase, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have the cytosolic isozyme but not the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of a catalytically active form of the mitochondrial isozyme. The increased exposure to acetaldehyde in individuals with the catalytically inactive form may also confer greater susceptibility to many types of cancer. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Mar 2011]
细胞定位Mitochondrion matrix.
研究领域Cell Biology and Cellular Response antibody; Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody
预测分子量56 kDa
检测图片 (4)
  • ARG55209 anti-ALDH2 antibody WB image

    Western blot: 30 μg of HepG2, A549, NIH-3T3, Mouse kidney, Mouse lung, Rat kidney and Rat lung lysates stained with ARG55209 anti-ALDH2 antibody at 1:500 dilution.

  • ARG55209 anti-ALDH2 antibody ICC/IF image

    Immunofluorescence: HepG2 cells stained with ARG55209 anti-ALDH2 antibody (green). DAPI (blue) for nuclear staining.

  • ARG55209 anti-ALDH2 antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human brain tissue stained with ARG55209 anti-ALDH2 antibody.

  • ARG55209 anti-ALDH2 antibody FACS image

    Flow Cytometry: HepG2 cells stained with ARG55209 anti-ALDH2 antibody (right histogram) or without primary antibody control (left histogram), followed by incubation with PE labelled secondary antibody.

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