anti-Aldolase C antibody [1A1]
![anti-Aldolase C antibody [1A1]](/upload/image/products/ARG10694_WB_2_210_205.jpg)
![anti-Aldolase C antibody [1A1]](/upload/image/products/ARG10694_ICC_1.jpg)
![anti-Aldolase C antibody [1A1]](/upload/image/products/ARG10694_WB_1.jpg)
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Key features and details
- 产品描述:
- 反应物种:
- 预测物种:
- 应用:
- 宿主:
- 克隆:
- 克隆号:
- 同位型:
- 靶点名称:
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Brand:
Product Details
| 产品描述 | Mouse Monoclonal antibody [1A1] recognizes Aldolase C |
|---|---|
| 反应物种 | Hu, Ms, Rat, Cow, Hrs, Pig |
| 预测物种 | Chk |
| 应用 | ICC/IF, IHC-Fr, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 1A1 |
| 同位型 | IgG1 |
| 靶点名称 | Aldolase C |
| 抗原 | C-terminal sequence KYEGSGEDGGAAAQSLYIANHAY |
| 偶联标记 | Un-conjugated |
| 別名 | EC 4.1.2.13; Brain-type aldolase; Fructose-bisphosphate aldolase C; ALDC |
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification. |
| 缓冲液 | PBS and 50% Glycerol. |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
| 数据库连接 | |
|---|---|
| 基因名称 | ALDOC |
| 全名 | aldolase C, fructose-bisphosphate |
| 背景介绍 | This gene encodes a member of the class I fructose-biphosphate aldolase gene family. Expressed specifically in the hippocampus and Purkinje cells of the brain, the encoded protein is a glycolytic enzyme that catalyzes the reversible aldol cleavage of fructose-1,6-biphosphate and fructose 1-phosphate to dihydroxyacetone phosphate and either glyceraldehyde-3-phosphate or glyceraldehyde, respectively. [provided by RefSeq, Jul 2008] |
| 预测分子量 | 39 kDa |
ARG10694 anti-Aldolase C antibody [1A1] ICC/IF image
Immunocytochemistry: Rat mixed neuron / glial cultures stained with ARG10694 anti-Aldolase C antibody [1A1] (green) and co-stained with rabbit antibody to NeuN/FOX3 antibody (RPCA-FOX3, red). MCA-1A1 antibody reveals strong cytoplasmic staining in astrocytes, while Rabbit Fox3/NeuN antibody shows nuclear and distal cytoplasmic staining in neuron cells and is complete absence of astrocytes. Blue is a DNA stain.
ARG10694 anti-Aldolase C antibody [1A1] WB image
Western blot: Crude Rat brain lysates stained with ARG10694 anti-Aldolase C antibody [1A1]. Other studies show that this antibody is completely specific for Aldolase C, and does not react with recombinant Human Aldolase A or B.
ARG10694 anti-Aldolase C antibody [1A1] WB image
Western blot: Recombinant Human Aldolase A, B and C with three monoclonal antibodies as indicated showing binding to all three gene products. Lane labelled S show molecular weight standards, while lanes A, B and C contain recombinant full length His-tagged Human Aldolase A, B and C respectively. The epitope for ARG10694 anti-Aldolase C antibody [1A1] is within the C-terminal peptide of the molecule which is less conserved between the three Aldolases. Clone 4A9 binds to the N-terminal peptide and also is specific for Aldolase C, while clone E9 binds to the less conserved core of the molecule and recognizes all three Aldolase C gene products.
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