anti-alpha A Crystallin antibody [1H3.B8]
![anti-alpha A Crystallin antibody [1H3.B8]](/upload/image/products/ARG22215_ICC-IF-Human-Neuroblastoma-cell-line.jpg)
![anti-alpha A Crystallin antibody [1H3.B8]](/upload/image/products/ARG22215_1.jpg)
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 宿主:
- 克隆:
- 克隆号:
- 同位型:
- 靶点名称:
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Brand:
Product Details
| 产品描述 | Mouse Monoclonal antibody [1H3.B8] recognizes alpha A Crystallin |
|---|---|
| 反应物种 | Hu, Ms, Rat, Bov |
| 应用 | ELISA, ICC/IF, WB |
| 特异性 | Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 1H3.B8 |
| 同位型 | IgG1 |
| 靶点名称 | alpha A Crystallin |
| 抗原物种 | Human |
| 抗原 | Native Alpha Crystallin |
| 偶联标记 | Un-conjugated |
| 別名 | CRYA1; HSPB4; CTRCT9 |
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS (pH 7.2), 0.09% Sodium azide and 50% Glycerol |
| 抗菌剂 | 0.09% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
| 数据库连接 | |
|---|---|
| 基因名称 | CRYAA |
| 全名 | crystallin alpha A |
| 细胞定位 | Cytoplasm, Nucleus |
| 产品亮点 | Related products: anti-alpha A Crystallin antibody [1H3.B8]; |
| 预测分子量 | 20 kDa |
ARG22215 anti-alpha A Crystallin antibody [1H3.B8] ICC/IF image
Immunofluorescence: Human Neuroblastoma cell line SK-N-BE. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: ARG22215 anti-alpha A Crystallin antibody [1H3.B8] at 1:100 for 60 min at RT. Secondary Antibody: Goat anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ARG22215 anti-alpha A Crystallin antibody [1H3.B8]. (D) Composite.
ARG22215 anti-alpha A Crystallin antibody [1H3.B8] WB image
Western blot: Bovine tissue lysate stained with ARG22215 anti-alpha A Crystallin antibody [1H3.B8] at 1:1000 dilution. This blot shows absolute specificity as Left: AlphaA Crystallin, Right: AlphaB Crystallin.
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