anti-alpha amylase antibody

Key features and details

  • 产品描述: Rabbit Polyclonal antibody recognizes alpha amylase.
  • 反应物种: Hu, Ms, Rat
  • 预测物种: Hm
  • 应用: IHC-P, WB
  • 特异性: Sequence blast result indicats this antibody might react to alpha amylase 1, alpha amylase 2B and pancreatic alpha amylase (AMY2A).
  • 宿主: Rabbit
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: alpha amylase
  • Brand:
CAT.NO. : ARG59664
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Product Details
概述
产品描述Rabbit Polyclonal antibody recognizes alpha amylase.
反应物种Hu, Ms, Rat
预测物种Hm
应用IHC-P, WB
特异性Sequence blast result indicats this antibody might react to alpha amylase 1, alpha amylase 2B and pancreatic alpha amylase (AMY2A).
宿主Rabbit
克隆Polyclonal
同位型IgG
靶点名称alpha amylase
抗原物种Human
抗原Synthetic peptide corresponding to aa. 30-44 of Human alpha amylase. (HLFEWRWVDIALECE)
偶联标记Un-conjugated
別名1,4-alpha-D-glucan glucanohydrolase 1; Salivary alpha-amylase; AMY1; EC 3.2.1.1; Alpha-amylase 1; PA; AMY2; HXA; AMY2; AMY3
应用说明
应用建议
应用推荐稀释比
IHC-P0.5 - 1 µg/ml
WB0.1 - 0.5 µg/ml
应用说明IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Affinity purification with immunogen.
缓冲液0.9% NaCl, 0.2% Na2HPO4, 0.05% Thimerosal, 0.05% Sodium azide and 5% BSA.
抗菌剂0.05% Thimerosal and 0.05% Sodium azide
稳定剂5% BSA
浓度0.5 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
基因名称AMY1A; AMY2A; AMY2B
全名amylase, alpha 1A (salivary); amylase, alpha 2A (pancreatic); amylase, alpha 2B (pancreatic)
背景介绍Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the salivary gland. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]
细胞定位Secreted. [UniProt]
预测分子量58 kDa
检测图片 (2)
  • ARG59664 anti-alpha amylase antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Rat spleen tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG59664 anti-alpha amylase antibody at 1 µg/ml, overnight at 4°C.

  • ARG59664 anti-alpha amylase antibody WB image

    Western blot: 50 µg of samples under reducing conditions. PANC-1, Rat pancreas and Mouse pancreas lysates stained with ARG59664 anti-alpha amylase antibody at 0.5 µg/ml, overnight at 4°C.

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